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Pal 蛋白, Legionella pneumophila (His-SUMO)

目录号: HY-P71467
COA 产品使用指南

Pal 蛋白是 Tol-Pal 系统的一部分,在细胞分裂过程中外膜内陷和维持完整性方面发挥着关键作用。该系统与 TolA、TolQ、TolR 和 TolB 集成,将内膜和外膜连接到肽聚糖层。Pal 与肽聚糖的紧密联系强调了它在 Tol-Pal 系统的结构和功能动力学中的关键作用。Pal 蛋白, Legionella pneumophila (His-SUMO) 是重组的 Pal 蛋白,由 E. coli 表达,带有 N-SUMO, N-6*His 标签。Pal 蛋白, Legionella pneumophila (His-SUMO) 全长 155 个氨基酸,相对电荷影响后的分子量为 ~36 kDa。

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描述

Pal protein, part of the Tol-Pal system, is pivotal in outer membrane invagination and maintenance of integrity during cell division. Integrated with TolA, TolQ, TolR, and TolB, the system connects inner and outer membranes to the peptidoglycan layer. Pal's strong association with peptidoglycan underscores its crucial role in the structural and functional dynamics of the Tol-Pal system. Pal Protein, Legionella pneumophila (His-SUMO) is the recombinant Pal protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of Pal Protein, Legionella pneumophila (His-SUMO) is 155 a.a., with molecular weight (affected by relative charge) of ~36 kDa.

研究背景

Pal 蛋白是 Tol-Pal 系统的重要组成部分,有助于细胞分裂过程中外膜内陷,并在维持外膜完整性方面发挥着至关重要的作用。该系统由五种核心蛋白(内膜中的 TolA、TolQ、TolR、周质中的 TolB 和外膜中的 Pal)组成,形成一个复杂的网络,将内膜、外膜与肽聚糖层连接起来。Pal 蛋白尤其表现出与肽聚糖的牢固关联,突出了其在 Tol-Pal 系统内结构和功能相互作用中的重要性。

种属

Others

表达系统

E. coli

标签

N-SUMO;N-6*His

蛋白编号

P26493 (C22-R176)

基因 ID

57036037

蛋白结构
N-term
6*His-SUMO
Pal (C22-R176)
Accession # P26493
C-term
同用名
pal; pplA; Peptidoglycan-associated lipoprotein; PAL; 19kDa surface antigen; PPL
氨基酸序列

CSKTPGSADGGAAVGDGDATAQGLGQMTHFAGQEPGESYTTQAPHNQLYLFAYDDSTLASKYLPSVNAQAEYLKTHPGARVMIAGHTDERGSREYNVALGERRADTVAEILRMAGVSRQQIRVVSYGKERPANYGHDEASHAQNRRVEFIYEATR

分子量

Approximately 36 kDa, The reducing (R) protein migrates as 36 kDa in SDS-PAGE may be due to relative charge.

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against solution in PBS, 6% Trehalose, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Pal Protein, Legionella pneumophila (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Pal Protein, Legionella pneumophila (His-SUMO)
目录号:
HY-P71467
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