PDK1 蛋白, Human (sf9, His)

PDK1 protein is a key kinase that controls glucose and fatty acid metabolism by phosphorylating pyruvate dehydrogenase subunits PDHA1 and PDHA2. This molecular switch inhibits pyruvate dehydrogenase, fine-tunes metabolite flux and downregulates aerobic respiration. PDK1 Protein, Human (sf9, His) is the recombinant human-derived PDK1 protein, expressed by Sf9 insect cells , with N-His labeled tag. The total length of PDK1 Protein, Human (sf9, His) is 408 a.a., with molecular weight of ~45 kDa.

PDK1 是一种关键激酶，通过磷酸化丙酮酸脱氢酶亚基 PDHA1 和 PDHA2 对葡萄糖和脂肪酸代谢发挥重要控制作用。这种磷酸化事件充当分子开关，抑制丙酮酸脱氢酶活性并微调三羧酸循环中的代谢物通量。因此，PDK1 下调有氧呼吸并阻碍丙酮酸转化为乙酰辅酶 A。除了其代谢功能外，PDK1 在细胞缺氧反应中发挥着关键作用，在缺氧条件下促进细胞增殖发挥着关键作用。条件匮乏。此外，PDK1 通过保护细胞免受缺氧和氧化应激引发的细胞凋亡来发挥保护作用，展示了其对细胞稳态和应激反应途径的多方面贡献。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-His

Q15118 (S29-A436)

5163  [NCBI]

Approximately 45 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 10% glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.