1. 重组蛋白
  2. Ubiquitin Related Proteins
  3. Ubiquitin Enzymes
  4. E3 Ligases
  5. Pellino-1
  6. Pellino-1 蛋白, Human (sf9, His-GST)

Pellino-1 蛋白, Human (sf9, His-GST)

目录号: HY-P75970
产品使用指南

Pellino-1 蛋白是一种 E3 泛素连接酶,可将泛素连接到底物蛋白。Pellino-1 蛋白, Human (sf9, His-GST) 是重组的 Pellino-1 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。Pellino-1 蛋白, Human (sf9, His-GST) 全长 418 个氨基酸,分子量约为 ~74.1 kDa。

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描述

Pellino-1 protein is an E3 ubiquitin ligase that links ubiquitin to substrate proteins. Pellino-1 Protein, Human (sf9, His-GST) is the recombinant human-derived Pellino-1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Pellino-1 Protein, Human (sf9, His-GST) is 418 a.a., with molecular weight of ~74.1 kDa.

研究背景

Pellino-1 充当 E3 泛素连接酶,协调泛素部分与底物蛋白的共价连接,特别是通过其相互作用在 Toll 样受体 (TLR) 和白细胞介素 1 (IL-1) 信号通路中发挥关键作用与含有IRAK激酶和TRAF6的复合物。值得注意的是,Pellino-1 介导 IRAK1 的“Lys-63”连接多泛素化,这是促进随后 NF-kappa-B 激活的关键步骤。此外,它在细胞命运决定中发挥调节作用,因为它催化 RIPK3 的“Lys-48”连接的多泛素化,导致其蛋白酶体依赖性降解,并优先选择 RIPK3 的“Thr-182”磷酸化形式。有趣的是,Pellino-1 通过其泛素连接酶活性下调 RIPK3 表达,从而负向调节坏死性凋亡。此外,Pellino-1 通过介导 RIPK1 的“Lys-63”连接泛素化来扩展其调节影响,从而有助于这些关键信号通路内细胞反应的复杂调节。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q96FA3 (M1-D418)

基因 ID
蛋白结构
N-term
GST-His
Pellino-1 (M1-D418)
Accession # Q96FA3
C-term
同用名
E3 ubiquitin-protein ligase pellino homolog 1; PELI1; PRISM
分子量

Approximately 74.1 kDa

纯度

Greater than 85% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

Pellino-1 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Pellino-1 Protein, Human (sf9, His-GST)
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HY-P75970
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