Peptide deformylase 蛋白, S. aureus (P.pastoris, His)

Peptide deformylase, a crucial enzyme in protein biosynthesis, catalyzes the removal of the formyl group from the N-terminal methionine of newly synthesized proteins. Displaying broad specificity at positions beyond the N-terminal L-methionine, the enzyme ensures proper maturation and functionality of proteins, emphasizing its essential contribution to the intricate process of protein synthesis and modification. Peptide deformylase Protein, S. aureus (P.pastoris, His) is the recombinant Staphylococcus aureus-derived Peptide deformylase protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of Peptide deformylase Protein, S. aureus (P.pastoris, His) is 183 a.a., with molecular weight of ~25 kDa.

肽去甲酰基酶是蛋白质生物合成中的关键酶，通过催化去除新合成蛋白质 N 末端甲硫氨酸的甲酰基，在细胞过程中发挥着关键作用。虽然它至少需要一个二肽才能获得最佳效率，但该酶在 N 端 L-甲硫氨酸以外的位置表现出广泛的特异性。这种活性确保了蛋白质的适当成熟和功能，突出了肽去酰基酶在蛋白质合成和修饰的复杂过程中的重要贡献。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Staphylococcus aureus

P. pastoris

N-6*His

P68826 (1M-183V)

/

MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNSQDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQEAYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLNGVMFYDHIDKNHPLQPHTDAVEV

Approximately 25 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.