1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PLA2G1B 蛋白, Mouse (HEK293, Fc)

PLA2G1B 蛋白, Mouse (HEK293, Fc)

目录号: HY-P78340
产品使用指南

PLA2G1B 蛋白是一种分泌型钙依赖性磷脂酶 A2,其目标是肠道中的膳食磷脂。优选磷脂酰乙醇胺和磷脂酰甘油,它们水解sn-2位脂肪酰基的酯键。PLA2G1B 蛋白, Mouse (HEK293, Fc) 是重组的 PLA2G1B 蛋白,由 HEK293 表达,带有 C-hFc 标签。PLA2G1B 蛋白, Mouse (HEK293, Fc) 全长 124 个氨基酸,分子量为 48-50 kDa。

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描述

The PLA2G1B protein is a secreted calcium-dependent phospholipase A2 that targets dietary phospholipids in the intestine. Phosphatidylethanolamine and phosphatidylglycerol are preferred, which hydrolyze the ester bond of the fatty acyl group at the sn-2 position. PLA2G1B Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived PLA2G1B protein, expressed by HEK293 , with C-hFc labeled tag. The total length of PLA2G1B Protein, Mouse (HEK293, Fc) is 124 a.a., with molecular weight of 48-50 kDa.

研究背景

PLA2G1B 是一种分泌型钙依赖性磷脂酶 A2,主要针对肠道中的膳食磷脂。它具有磷脂酶 A2 活性,可水解磷脂 sn-2 位脂肪酰基的酯键,优先水解磷脂酰乙醇胺和磷脂酰甘油而不是磷脂酰胆碱。PLA2G1B 可能在 N-酰基乙醇胺的生物合成中发挥至关重要的作用,调节肠道能量代谢和炎症。它以自分泌和旁分泌方式发挥作用,与 PLA2R1 受体结合后,可以调节足细胞存活和肾小球稳态。此外,PLA2G1B 具有抗蠕虫活性,在肠道上皮感染(肠道微生物群调节的过程)过程中直接影响蠕虫幼虫膜中磷脂酰乙醇胺的含量,从而影响蠕虫幼虫的完整性和传染性。

种属

Mouse

表达系统

HEK293

标签

C-hFc

蛋白编号

Q9Z0Y2 (A23-C146)

基因 ID

18778  [NCBI]

蛋白结构
N-term
PLA2G1B (A23-C146)
Accession # Q9Z0Y2
hFc
C-term
同用名
Phospholipase A2; PLA2G1B; PLA2; PLA2A; PLA2AMGC119834
分子量

48-50 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PLA2G1B Protein, Mouse (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PLA2G1B Protein, Mouse (HEK293, Fc)
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