PLA2G2A 蛋白, Human (HEK293, His)

The PLA2G2A protein is a secreted calcium-dependent phospholipase A2 that selectively targets extracellular phospholipids and contributes to host antimicrobial defense, inflammation, and tissue regeneration. It has phospholipase A2 activity, which preferentially hydrolyzes fatty acyl groups, phosphatidylethanolamine and phosphatidylglycerol. PLA2G2A Protein, Human (HEK293, His) is the recombinant human-derived PLA2G2A protein, expressed by HEK293 , with C-His labeled tag. The total length of PLA2G2A Protein, Human (HEK293, His) is 144 a.a., with molecular weight of ~19 kDa.

PLA2G2A 是一种分泌型钙依赖性磷脂酶 A2，选择性地靶向细胞外磷脂，对宿主抗菌防御、炎症反应和组织再生具有重要意义。它利用磷脂酶 A2 活性，水解磷脂 sn-2 位脂肪酰基的酯键，与磷脂酰胆碱相比，优先选择磷脂酰乙醇胺和磷脂酰甘油。PLA2G2A 积极促进细胞膜中的脂质重塑以及对病原体清除至关重要的脂质介质的产生，对革兰氏阳性菌发挥杀菌活性。在无菌炎症期间，它以细胞外线粒体的膜磷脂为目标，释放游离的不饱和脂肪酸，如花生四烯酸。这种花生四烯酸被邻近的白细胞利用来合成炎症类二十烷酸，例如白三烯。同时，通过损害线粒体膜的完整性，PLA2G2A 促进有效的损伤相关分子模式分子释放到循环中，激活先天免疫反应。在肠隐窝中，PLA2G2A 作为干细胞调节剂，介导潘氏细胞分化并通过抑制 Wnt 信号通路支持干细胞功能。炎症期间在肠腔中分泌，以自分泌方式发挥作用，促进前列腺素 E2 合成，刺激肠道干细胞中的 Wnt 信号通路，有助于组织再生。此外，PLA2G2A 可能在 N-酰基乙醇胺的生物合成中发挥作用，调节能量代谢和炎症，并且通过不同的途径结合并激活整合素 ITGAV:ITGB3、ITGA4:ITGB1 和 ITGA5:ITGB1，从而表现出整合素依赖性细胞增殖。配体结合位点。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

HEK293

C-His

P14555 (M1-C144)

5320  [NCBI]

Approximately 19 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.