2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. PLBD2 蛋白, Mouse (His)

PLBD2 蛋白, Mouse (His)

目录号: HY-P71553
产品使用指南

PLBD2 蛋白是一种假定的磷脂酶，与胰岛素样生长因子 2 受体 (IGF2R) 相互作用，表明其参与生长和信号传导通路。尽管有关其磷脂酶活性的细节尚不清楚，但 PLBD2 的探索可能揭示其在细胞稳态和调节机制中的作用。PLBD2 蛋白, Mouse (His) 是重组的 PLBD2 蛋白，由 E. coli 表达，带有 N-His 标签。PLBD2 蛋白, Mouse (His) 全长 548 个氨基酸，分子量约为 ~65.9 kDa。

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描述

PLBD2 Protein, a putative phospholipase, interacts with insulin-like growth factor 2 receptor (IGF2R), suggesting involvement in growth and signaling pathways. Despite unknown details about its phospholipase activity, PLBD2's exploration may unveil insights into its role in cellular homeostasis and regulatory mechanisms. PLBD2 Protein, Mouse (His) is the recombinant mouse-derived PLBD2 protein, expressed by E. coli , with N-His labeled tag. The total length of PLBD2 Protein, Mouse (His) is 548 a.a., with molecular weight of ~65.9 kDa.
研究背景

PLBD2 蛋白被认为是一种假定的磷脂酶，与胰岛素样生长因子 2 受体 (IGF2R) 具有相互作用。其磷脂酶活性的精确性质及其在细胞过程中的潜在影响仍然是人们感兴趣的领域，因为该蛋白质与 IGF2R 的相互作用表明其在与生长和信号传导相关的途径中发挥作用。进一步探索 PLBD2 的功能特征可能有助于了解其对细胞稳态和调节机制的贡献。
种属

Mouse
表达系统

E. coli
标签

N-His
蛋白编号

Q3TCN2 (47L-594D)
基因 ID

71772  [NCBI]
蛋白结构
N-term
His
PLBD2 (47L-594D)
Accession # Q3TCN2
C-term
同用名
Plbd2; Putative phospholipase B-like 2; EC 3.1.1.-; 66.3kDa protein; 76kDa protein; p76; LAMA-like protein 2; Lamina ancestor homolog 2
氨基酸序列

LPTLGPGWQRQNPDPPVSRTRSLLLDAASGQLRLEDGFHPDAVAWANLTNAIRETGWAYLDLSTNGRYNDSLQAYAAGVVEASVSEELIYMHWMNTVVNYCGPFEYEVGYCEKLKNFLEANLEWMQREMELNPDSPYWHQVRLTLLQLKGLEDSYEGRLTFPTGRFTIKPLGFLLLQISGDLEDLEPALNKTNTKPSLGSGSCSALIKLLPGGHDLLVAHNTWNSYQNMLRIIKKYRLQFREGPQEEYPLVAGNNLVFSSYPGTIFSGDDFYILGSGLVTLETTIGNKNPALWKYVQPQGCVLEWIRNVVANRLALDGATWADVFKRFNSGTYNNQWMIVDYKAFLPNGPSPGSRVLTILEQIPGMVVVADKTAELYKTTYWASYNIPYFETVFNASGLQALVAQYGDWFSYTKNPRAKIFQRDQSLVEDMDAMVRLMRYNDFLHDPLSLCEACNPKPNAENAISARSDLNPANGSYPFQALHQRAHGGIDVKVTSFTLAKYMSMLAASGPTWDQCPPFQWSKSPFHSMLHMGQPDLWMFSPIRVPWD

分子量

Approximately 65.9 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

PLBD2 Protein, Mouse (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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