1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. PLD4 蛋白, Mouse (HEK293, His)

PLD4 蛋白, Mouse (HEK293, His)

目录号: HY-P77821
产品使用指南

PLD4 蛋白具有 5'->3' DNA 核酸外切酶活性,可有效消化单链 DNA (ssDNA)。它通过降解核酸、降低激活 TLR9 的 ssDNA 浓度来至关重要地调节炎症细胞因子反应。此外,PLD4 积极参与活化小胶质细胞的吞噬过程,强调其在免疫反应中的作用。PLD4 蛋白, Mouse (HEK293, His) 是重组的 PLD4 蛋白,由 HEK293 表达,带有 C-His 标签。PLD4 蛋白, Mouse (HEK293, His) 全长 446 个氨基酸,分子量为 60-80 kDa。

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     可免费申领三个不同产品的试用装。

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描述

PLD4 Protein, with 5'->3' DNA exonuclease activity, efficiently digests single-stranded DNA (ssDNA). It crucially regulates inflammatory cytokine responses by degrading nucleic acids, reducing ssDNA concentrations activating TLR9. Additionally, PLD4 actively participates in the phagocytosis process of activated microglia, emphasizing its role in immune responses. PLD4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived PLD4 protein, expressed by HEK293, with C-His labeled tag. The total length of PLD4 Protein, Mouse (HEK293, His) is 446 a.a., with molecular weight of 60-80 kDa.

研究背景

PLD4 蛋白表现出 5'->3' DNA 核酸外切酶活性,使其能够有效消化单链 DNA (ssDNA)。它通过降解核酸从而降低可激活 TLR9(一种核苷酸敏感受体)的 ssDNA 浓度,在调节炎症细胞因子反应中发挥至关重要的作用。此外,PLD4 积极参与活化小胶质细胞的吞噬过程,突显其在免疫反应和细胞功能中的重要性。

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

Q8BG07 (Q58-W503)

基因 ID

104759  [NCBI]

蛋白结构
N-term
PLD4 (Q58-W503)
Accession # Q8BG07
His
C-term
同用名
5'-3' exonuclease PLD4; Choline phosphatase 4; Phospholipase D4; PLD 4
分子量

60-80 kDa

纯度

Greater than 95% as determined by Tris-Bis PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PLD4 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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PLD4 Protein, Mouse (HEK293, His)
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