PSMA3 蛋白, Human (His)

PSMA3 is a protein involved in protein degradation. PSMA3 Protein, Human (His) is the recombinant human-derived PSMA3 protein, expressed by E. coli , with N-His labeled tag. The total length of PSMA3 Protein, Human (His) is 255 a.a., with molecular weight of ~30 kDa.

PSMA3 是 20S 核心蛋白酶体复合物的重要组成部分，在泛素化蛋白质的 ATP 依赖性降解中发挥着关键作用，有助于维持蛋白质稳态。当与两个 19S 调节颗粒结合时，它会形成 26S 蛋白酶体，它对于去除错误折叠或受损的蛋白质以保护细胞功能至关重要。20S 蛋白酶体与 PA200 或 PA28 结合，促进不依赖于泛素的蛋白质降解，这对于精子发生和 MHC I 类呈递抗原肽的生成等过程至关重要。PSMA3 还与 CDKN1A 的 C 末端结合，介导其降解，并负向调节细胞表面血栓素 A2 受体亚型 2 的膜运输。26S 蛋白酶体由 20S 蛋白酶体核心和两个 19S 调节亚基组成，充当桶状复合体由排列成四个堆叠环的 28 个亚基组成。20S 核心的蛋白水解活性由三个 β 亚基 PSMB5、PSMB6 和 PSMB7 执行。PSMA3 进一步参与与 AURKB、CDKN1A、MDM2、RB1、TBXA2R 同工型 2 和 DNAJB2 的蛋白质相互作用，强调了其在细胞调节途径中的多功能作用。

Measured by its ability to inhibit the proliferation of SK-OV-3 cells. The ED₅₀ for this effect is 0.1116 μg/mL, Corresponding to a specific activity is 8.960×10³ Unit/mg.

Human

E. coli

N-6*His

P25788-1 (M1-M255)

5684  [NCBI]

MSSIGTGYDLSASTFSPDGRVFQVEYAMKAVENSSTAIGIRCKDGVVFGVEKLVLSKLYEEGSNKRLFNVDRHVGMAVAGLLADARSLADIAREEASNFRSNFGYNIPLKHLADRVAMYVHAYTLYSAVRPFGCSFMLGSYSVNDGAQLYMIDPSGVSYGYWGCAIGKARQAAKTEIEKLQMKEMTCRDIVKEVAKIIYIVHDEVKDKAFELELSWVGELTNGRHEIVPKDIREEAEKYAKESLKEEDESDDDNM

Approximately 30 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.