S100A4 蛋白, Human (C-His)

The S100A4 protein is a calcium-binding protein involved in angiogenesis, cell differentiation, apoptosis, and autophagy. S100A4 protein interacts with MYH9 to enhance cell motility and invasion. S100A4 also inhibits the pro-apoptotic function of TP53 by reducing its protein levels. S100A4 Protein, Human (C-His) is the recombinant human-derived S100A4 protein, expressed by E. coli , with C-6*His labeled tag. The total length of S100A4 Protein, Human (C-His) is 101 a.a., with molecular weight of ~13.0 kDa.

S100A4 蛋白是一种钙结合分子，在许多细胞过程中发挥着多种作用，包括运动、血管生成、细胞分化、细胞凋亡和自噬。在功能上，它通过与非肌肉肌球蛋白重链 IIA/MYH9 相互作用，促进肌丝解聚并增加可溶性肌球蛋白-IIA 的量以促进趋化性，从而增强细胞运动性和侵袭性。此外，S100A4 通过与细胞核内的 C 端反式激活结构域结合来调节 TP53 的促凋亡功能，从而导致 TP53 蛋白水平降低。在细胞外空间，它刺激细胞因子的产生，并与 PGLYRP1 形成趋化复合物，通过 CCR5 和 CXCR3 受体促进淋巴细胞迁移。该蛋白质形成同二聚体并与各种伙伴相互作用，包括 PPFIBP1、膜联蛋白 2/ANXA2、TP53、CCR5 和 FCGR3A，每种相互作用都有助于其多样化的细胞功能。这种复杂的相互作用网络强调了 S100A4 在协调不同途径的细胞反应中的多方面作用。

Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 0.5755 ng/mL , corresponding to a specific activity is 1.73×10⁶ units/mg.

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  Measured in a cell proliferation assay using A549 cells. The ED₅₀ for this effect is 0.5755 ng/mL , corresponding to a specific activity is 1.73×10⁶ units/mg.

Human

E. coli

C-6*His

P26447 (M1-K101)

6275  [NCBI]

MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK

Approximately 13.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.