Semaphorin-4D/SEMA4D 蛋白, Mouse (HEK293, Fc)

Semaphorin-4D/SEMA4D is the cell surface receptor for PLXNB1 and PLXNB2 and plays a crucial role in intercellular signaling. It regulates GABAergic synapse development and promotes inhibitory synapses in a PLXNB1-dependent manner. Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Semaphorin-4D/SEMA4D protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Semaphorin-4D/SEMA4D Protein, Mouse (HEK293, Fc) is 688 a.a., with molecular weight of 100-130 kDa.

Semaphorin-4D (SEMA4D) 作为 PLXNB1 和 PLXNB2 的细胞表面受体，在细胞间信号传导中发挥着至关重要的作用。这种多功能蛋白质涉及多种细胞过程，包括 GABA 能突触发育的调节，它以 PLXNB1 依赖性方式促进抑制性突触的发育。在海马神经元中，SEMA4D 通过激活 PLXNB1 影响发育中神经突的复杂性和树枝化，从而介导 RHOA 激活。此外，SEMA4D 还参与促进小脑颗粒细胞的迁移并诱导 B 细胞聚集，从而增强其体外活力。此外，SEMA4D 通过激活 PTK2B/PYK2、SRC 和磷脂酰肌醇 3-激酶-AKT 途径刺激内皮细胞迁移。作为同二聚体，SEMA4D 与 PLXNB1 和 PLXNB2 相互作用，强调了它在各种细胞和发育过程中的复杂参与。

Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ this effect is 0.4301 μg/mL, corresponding to a specific activity is 2.33×10³ units/mg.

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  Measured in a cell proliferation assay using MDA-MB-231 cells. The ED₅₀ for this effect is 0.4301 μg/mL, corresponding to a specific activity is 2.33×10³ units/mg.

Mouse

HEK293

C-hFc

O09126 (F24-M711)

20354  [NCBI]

FAPVPRLTWEHGEVGLVQFHKPGIFNYSALLMSEDKDTLYVGAREAVFAVNALNISEKQHEVYWKVSEDKKSKCAEKGKSKQTECLNYIRVLQPLSSTSLYVCGTNAFQPTCDHLNLTSFKFLGKSEDGKGRCPFDPAHSYTSVMVGGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIQKSPDGPEGEDDKVYFFFTEVSVEYEFVFKLMIPRVARVCKGDQGGLRTLQKKWTSFLKARLICSKPDSGLVFNILQDVFVLRAPGLKEPVFYAVFTPQLNNVGLSAVCAYTLATVEAVFSRGKYMQSATVEQSHTKWVRYNGPVPTPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPKLIKKDVNYTQIVVDRTQALDGTFYDVMFISTDRGALHKAVILTKEVHVIEETQLFRDSEPVLTLLLSSKKGRKFVYAGSNSGVVQAPLAFCEKHGSCEDCVLARDPYCAWSPAIKACVTLHQEEASSRGWIQDMSGDTSSCLDKSKESFNQHFFKHGGTAELKCFQKSNLARVVWKFQNGELKAASPKYGFVGRKHLLIFNLSDGDSGVYQCLSEERVRNKTVSQLLAKHVLEVKMVPRTPPSPTSEDAQTEGSKITSKMPVASTQGSSPPTPALWATSPRAATLPPKSSSGTSCEPKM

100-130 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB,150 mM NaCl, pH 7.4 or PBS.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.