SHH 蛋白, Human (HEK293, His)

The SHH protein is cleaved into ShhN and ShhC fragments through autoproteolysis and cholesterol transferase activity. In the endoplasmic reticulum, ShhC is degraded, leaving behind lipidated ShhNp that is critical for developmental patterning. SHH Protein, Human (HEK293, His) is the recombinant human-derived SHH protein, expressed by HEK293 , with C-His labeled tag. The total length of SHH Protein, Human (HEK293, His) is 197 a.a., with molecular weight of ~24 kDa.

音刺猬 (SHH) 蛋白前体在其 C 末端部分经历自蛋白水解和胆固醇转移酶活性，导致全长蛋白裂解成两个片段：ShhN 和 ShhC，其中胆固醇部分共价连接到 C 端。-新生成的ShhN的终点。这些过程发生在内质网中，ShhC 随后在内质网中被降解。双脂化音刺猬蛋白 N-产物 (ShhNp) 作为发育过程中多种模式形成事件所必需的形态发生素。ShhNp 诱导神经管和体节中的腹侧细胞命运，参与发育中的肢芽的前后轴模式，并在轴突引导中发挥至关重要的作用。SHH 与 patched (PTCH1) 受体结合，与 smoothened (SMO) 受体结合，激活靶基因的转录。在缺乏 SHH 的情况下，PTCH1 抑制 SMO 的组成型信号活动。

Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells and the ED₅₀ is typically 1-10 μg/mL.

Human

HEK293

C-His

Q15465 (M1-G197)

6469  [NCBI]

Approximately 24 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.