SIRT1 蛋白, Human (746a.a, His)

SIRT1 is an NAD-dependent protein deacetylase that integrates multiple cellular functions—cell cycle progression, DNA damage response, metabolism, apoptosis, and autophagy—by regulating the acetylation of various target proteins. SIRT1 responds to changes in the NAD(+)/NADH ratio caused by factors such as glucose deprivation, affecting chromatin remodeling through histone deacetylation, resulting in transcriptional regulation. SIRT1 Protein, Human (746a.a, His) is the recombinant human-derived SIRT1 protein, expressed by E. coli , with N-6*His labeled tag. The total length of SIRT1 Protein, Human (746a.a, His) is 746 a.a., with molecular weight of ~90 kDa.

SIRT1 是一种 NAD 依赖性蛋白脱乙酰酶，通过调节众多靶蛋白的乙酰化状态，协调各种细胞功能的整合，包括细胞周期进程、对 DNA 损伤的反应、代谢、细胞凋亡和自噬。SIRT1 作为 NAD(+)/NADH 比率的传感器，对葡萄糖剥夺和热量限制等因素引起的细胞能量变化做出反应。其广泛的影响包括通过组蛋白脱乙酰化进行染色质重塑，最终导致转录调控。SIRT1 参与多种相互作用，使转录因子、共调节子和组蛋白去乙酰化，从而对靶基因表达发挥正向和负向调节作用。此外，SIRT1 还涉及 DNA 损伤反应、自噬、昼夜节律调节和代谢稳态等关键过程。其多方面的作用延伸到各种细胞途径的调节和基因组完整性的维持，突出了其在细胞生理学和应激反应中的重要性。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q96EB6 (2A-747S)

23411  [NCBI]

ADEAALALQPGGSPSAAGADREAASSPAGEPLRKRPRRDGPGLERSPGEPGGAAPEREVPAAARGCPGAAAAALWREAEAEAAAAGGEQEAQATAAAGEGDNGPGLQGPSREPPLADNLYDEDDDDEGEEEEEAAAAAIGYRDNLLFGDEIITNGFHSCESDEEDRASHASSSDWTPRPRIGPYTFVQQHLMIGTDPRTILKDLLPETIPPPELDDMTLWQIVINILSEPPKRKKRKDINTIEDAVKLLQECKKIIVLTGAGVSVSCGIPDFRSRDGIYARLAVDFPDLPDPQAMFDIEYFRKDPRPFFKFAKEIYPGQFQPSLCHKFIALSDKEGKLLRNYTQNIDTLEQVAGIQRIIQCHGSFATASCLICKYKVDCEAVRGDIFNQVVPRCPRCPADEPLAIMKPEIVFFGENLPEQFHRAMKYDKDEVDLLIVIGSSLKVRPVALIPSSIPHEVPQILINREPLPHLHFDVELLGDCDVIINELCHRLGGEYAKLCCNPVKLSEITEKPPRTQKELAYLSELPPTPLHVSEDSSSPERTSPPDSSVIVTLLDQAAKSNDDLDVSESKGCMEEKPQEVQTSRNVESIAEQMENPDLKNVGSSTGEKNERTSVAGTVRKCWPNRVAKEQISRRLDGNQYLFLPPNRYIFHGAEVYSDSEDDVLSSSSCGSNSDSGTCQSPSLEEPMEDESEIEEFYNGLEDEPDVPERAGGAGFGTDGDDQEAINEAISVKQEVTDMNYPSNKS

Approximately 90 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.