1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TGF-beta Superfamily
  4. Smad Family
  5. SMAD2
  6. SMAD2 蛋白, Mouse (sf9, His-GST)

SMAD2 蛋白, Mouse (sf9, His-GST)

目录号: HY-P73637
产品使用指南

SMAD2 蛋白与多个伙伴相互作用,例如 CREB 结合蛋白 (CBP)、EP300、SNON 等。这些相互作用有助于不同的细胞过程,包括 TGF-β 信号传导和转录调节。SMAD2 蛋白, Mouse (sf9, His-GST) 是重组的 SMAD2 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。SMAD2 蛋白, Mouse (sf9, His-GST) 全长 466 个氨基酸,分子量约为 ~90 kDa。

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2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

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描述

SMAD2 protein interacts with multiple partners, such as CREB-binding protein (CBP), EP300, SNON, and others. These interactions contribute to diverse cellular processes, including TGF-beta signaling and transcriptional regulation. SMAD2 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived SMAD2 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of SMAD2 Protein, Mouse (sf9, His-GST) is 466 a.a., with molecular weight of ~90 kDa.

研究背景

CREB 结合蛋白 (CBP) 与其他几种蛋白相互作用,包括 EP300、SNON、ALK4/ACVR1B、SKOR1、SKOR2、PRDM16、LEMD3、RBPMS、WWP1、RANBP3、PDPK1、DAB2、USP15、PPP5C、LDLRAD4、PMEPA1、 ZFHX3、ZNF451、SMURF2、PPM1A、TGF-β、TGFBR1、TGIF、SMAD3、TRIM33、ZNF580、NEDD4L、HGS、AIP1、WWP1、PML、ZNF8、RNF111、YAP1 和 IPO7。这些相互作用在各种细胞过程中发挥作用,包括 TGF-β 信号传导和转录调节。

种属

Mouse

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q62432 (S2-S467)

基因 ID

17126  [NCBI]

蛋白结构
N-term
His-GST
SMAD2 (S2-S467)
Accession # Q62432
C-term
同用名
Mothers against decapentaplegic homolog 2; MAD homolog 2; hMAD-2; SMAD2; MADH2; MADR2
分子量

Approximately 90 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SMAD2 Protein, Mouse (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SMAD2 Protein, Mouse (sf9, His-GST)
目录号:
HY-P73637
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