SNCA 蛋白, Mouse

SNCA proteins are critical for synaptic activity and regulate vesicle trafficking and neurotransmitter release. As a monomer, it enhances the vesicle exocytosis process. SNCA Protein, Mouse is the recombinant mouse-derived SNCA protein, expressed by E. coli , with tag free. The total length of SNCA Protein, Mouse is 140 a.a., with molecular weight of ~17.0 kDa.

SNCA 蛋白是突触活动的关键参与者，在调节突触小泡运输和神经递质释放中发挥多种作用。它在突触小泡胞吐作用中充当单体，增强囊泡启动、融合和胞吐融合孔扩张等过程。此外，SNCA 在其多聚体膜结合状态下充当分子伴侣，协助突触前质膜上称为 SNARE 的突触融合成分的折叠。这种伴侣活性对于在衰老过程中维持正常的 SNARE 复合体组装至关重要。SNCA 还通过与多巴胺转运蛋白 (DAT1) 相互作用并调节其活性来调节多巴胺神经传递。SNCA 以可溶性单体和同四聚体的形式存在，动态平衡四聚体和单体，其中四聚体在体内平衡中发挥着至关重要的作用。SNCA 与 UCHL1、synphilin-1/SNCAIP、CALM1、STXBP1、VAMP2、SNAP25、RPH3A、RAB3A、SERF1A 和 SEPTIN4 等蛋白质进行各种相互作用，说明其参与控制突触功能和完整性的复杂分子网络。

Immobilized Mouse Alpha-Synuclein at 1 μg/mL (100 μL/well) can bind Anti-Synucelin alpha/beta Monoclonal Antibody, the ED₅₀ for this effect is 316.4 ng/mL.

Mouse

E. coli

Tag Free

O55042 (M1-A140)

20617  [NCBI]

MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVTTVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGNIAAATGFVKKDQMGKGEEGYPQEGILEDMPVDPGSEAYEMPSEEGYQDYEPEA

Approximately 17.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.