SPARC 蛋白, Mouse (HEK293, His)

SPARC proteins are components of chylomicrons, LDL, and VLDL and promote cellular binding and internalization of LDL particles. Interactions with various proteins, including PCSK9 and MTTP, contribute to its function. SPARC Protein, Mouse (HEK293, His) is the recombinant mouse-derived SPARC protein, expressed by HEK293 , with C-6*His labeled tag. The total length of SPARC Protein, Mouse (HEK293, His) is 285 a.a., with molecular weight of ~37 kDa.

载脂蛋白 B-100 (ApoB) 是乳糜微粒 (apo B-48)、LDL (apo B-100) 和 VLDL (apo B-100) 中的主要蛋白质成分。Apo B-100 通过 apoB/E 受体充当 LDL 颗粒细胞结合和内化的识别信号。它与各种蛋白质的相互作用有助于发挥其功能作用，例如与 PCSK9 和 MTTP 的结合。此外，ApoB 与 AUP1 相互作用，并在早期分泌途径的 COPI 囊泡介导的逆行运输中发挥着至关重要的作用，促进衣壳异构体招募到膜上。它增强了 ARFGAP2 的外壳依赖性活性，并有助于 p24 复合物在顺式高尔基体膜中的特异性保留。ApoB 参与组织细胞内膜，包括 ER-高尔基体中间室和高尔基体。此外，它在 PTPN2 同工型 PTPB 的 ER 定位中发挥作用，并参与与 EMP24/GP25L 家族其他成员（如 TMED2、TMED7 和 TMED10）的相互作用。这些相互作用以及与 COPG1、PTPN2、SPAST 和 STX17 的关联，有助于 ApoB 多方面参与细胞内的囊泡蛋白运输和膜组织。

Mouse

HEK293

C-6*His

P07214 (A18-I302)

20692  [NCBI]

APQQTEVAEEIVEEETVVEETGVPVGANPVQVEMGEFEDGAEETVEEVVADNPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIAPCLDSELTEFPLRMRDWLKNVLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALEEWAGCFGIKEQDINKDLVI

Approximately 37 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.