SSB 蛋白, Human

The SSB protein critically binds to the 3' poly(U) terminus of RNA polymerase III transcripts, preventing exonuclease digestion and aiding in correct folding. In coxsackievirus B3 infection, SSB binds to the viral internal ribosome entry site (IRES) and enhances IRES-mediated translation. SSB Protein, Human is the recombinant human-derived SSB protein, expressed by E. coli , with tag free. The total length of SSB Protein, Human is 408 a.a., with molecular weight of ~50 kDa.

SSB 蛋白通过结合新生 RNA 聚合酶 III 转录物的 3' Poly(U) 末端发挥着至关重要的作用，提供针对核酸外切酶消化的保护并促进其正确折叠和成熟，正如各种研究所证明的那样。此外，在柯萨奇病毒 B3 感染的情况下，SSB 与病毒内部核糖体进入位点 (IRES) 结合，从而刺激 IRES 介导的翻译。该蛋白进一步与 DDX15 相互作用，并可能与 RUFY1 相互作用，表明它参与多种细胞过程并强调其多功能性质。

Data is not available.

Human

E. coli

Tag Free

P05455 (M1-Q408)

6741  [NCBI]

MAENGDNEKMAALEAKICHQIEYYFGDFNLPRDKFLKEQIKLDEGWVPLEIMIKFNRLNRLTTDFNVIVEALSKSKAELMEISEDKTKIRRSPSKPLPEVTDEYKNDVKNRSVYIKGFPTDATLDDIKEWLEDKGQVLNIQMRRTLHKAFKGSIFVVFDSIESAKKFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ

Approximately 50 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized a 0.2 μm filtered solution of PBS, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.