1. 重组蛋白
  2. Enzymes & Regulators
  3. Serine/Threonine Kinase Proteins
  4. TAO Kinase 3
  5. TAOK3 蛋白, Human (sf9, His-GST)

TAOK3 蛋白, Human (sf9, His-GST)

目录号: HY-P77222
产品使用指南

TAOK3 是一种激酶,调节 p38/MAPK14 和 MAPK8/JNK 级联。它可能通过 MAP2K3 和 MAP2K6 磷酸化激活 p38/MAPK14 级联,特别是在 DNA 损伤诱导的 G2/M 转变中。TAOK3 抑制基础 MAPK8/JNK 活性并抑制其对表皮生长因子的反应。这些功能展示了 TAOK3 在协调细胞对 DNA 损伤和生长因子信号传导的反应中的作用。TAOK3 蛋白, Human (sf9, His-GST) 是重组的 TAOK3 蛋白,由 Sf9 insect cells 表达,带有 N-His, N-GST 标签。TAOK3 蛋白, Human (sf9, His-GST) 全长 411 个氨基酸,分子量约为 ~85 kDa。

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描述

TAOK3, a kinase, regulates the p38/MAPK14 and MAPK8/JNK cascades. It activates the p38/MAPK14 cascade, particularly in DNA damage-induced G2/M transition, likely through MAP2K3 and MAP2K6 phosphorylation. TAOK3 inhibits basal MAPK8/JNK activity and dampens its response to epidermal growth factor. These functions showcase TAOK3's role in coordinating cellular responses to DNA damage and growth factor signaling. TAOK3 Protein, Human (sf9, His-GST) is the recombinant human-derived TAOK3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of TAOK3 Protein, Human (sf9, His-GST) is 411 a.a., with molecular weight of ~85 kDa.

研究背景

TAOK3 是一种丝氨酸/苏氨酸蛋白激酶,作为 p38/MAPK14 应激激活的 MAPK 级联和 MAPK8/JNK 级联的调节器。它作为 p38/MAPK14 级联的激活剂,特别是在响应 DNA 损伤时,通过激活 p38/MAPK14 级联在 G2/M 转变 DNA 损伤检查点中发挥作用。这种激活可能是通过上游 MAP2K3 和 MAP2K6 激酶的磷酸化介导的。此外,TAOK3 作为 MAPK8/JNK 级联基础活性的抑制剂,并减少其响应表皮生长因子 (EGF) 的激活。这些调节功能凸显了 TAOK3 在协调细胞对 DNA 损伤和生长因子信号传导反应中的作用。

种属

Human

表达系统

Sf9 insect cells

标签

N-His;N-GST

蛋白编号

Q9H2K8 (M1-D411)

基因 ID
蛋白结构
N-term
His-GST
TAOK3 (M1-D411)
Accession # Q9H2K8
C-term
同用名
Serine/threonine-protein kinase TAO3; JNK/SAPK-inhibitory kinase; DPK; JIK; KDS; MAP3K18
分子量

Approximately 85 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

TAOK3 Protein, Human (sf9, His-GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TAOK3 Protein, Human (sf9, His-GST)
目录号:
HY-P77222
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