2. 重组蛋白
  3. Cytokines and Growth Factors
  4. TFF3 蛋白, Canine (HEK293, His)

TFF3 蛋白, Canine (HEK293, His)

目录号: HY-P700527
产品使用指南

TFF3 蛋白复杂地维护和修复肠粘膜，作为一种运动原，在愈合过程中促进上皮细胞的移动性。TFF3 作为单体存在，可以通过二硫键形成同二聚体，这表明 TFF3 在介导关键相互作用中发挥着作用。它参与粘膜修复，强调了 TFF3 在保持肠内壁完整性和功能的动态过程中的重要性。TFF3 蛋白, Canine (HEK293, His) 是重组的 TFF3 蛋白，由 HEK293 表达，带有 C-10*His 标签。TFF3 蛋白, Canine (HEK293, His) 全长 57 个氨基酸，分子量为 9.2 kDa。

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描述

TFF3 Protein intricately maintains and repairs the intestinal mucosa, acting as a motogen that promotes epithelial cell mobility during healing. Existing as a monomer, TFF3 can form homodimers through disulfide linkages, suggesting a role in mediating crucial interactions. Its involvement in mucosal repair underscores TFF3's significance in dynamic processes that preserve the integrity and functionality of the intestinal lining. TFF3 Protein, Canine (HEK293, His) is the recombinant canine-derived TFF3 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of TFF3 Protein, Canine (HEK293, His) is 57 a.a., with molecular weight of 9.2 kDa.
研究背景

TFF3 蛋白复杂地参与肠粘膜的维护和修复，在愈合过程中促进上皮细胞的活动性方面发挥着关键作用，充当运动原。该蛋白质以单体形式存在，可以通过二硫键形成同二聚体。这种二聚体结构强调了其在介导对其功能活动至关重要的相互作用中的潜在作用。TFF3 参与粘膜修复凸显了其在维持肠内壁完整性和功能的动态过程中的重要性。
种属

Canine
表达系统

HEK293
标签

C-10*His
蛋白编号

Q863B4 (Y24-F80)
基因 ID

403488  [NCBI]
蛋白结构
N-term
TFF3 (Y24-F80)
Accession # Q863B4
10*His
C-term
同用名
TFF3; trefoil factor 3 (intestinal); ITF; Polypeptide P1.B; TFI; HITF; trefoil factor 3, HITF, human intestinal trefoil factor; OTTHUMP00000109349; P1B; trefoil factor 3; hITF; hP1.B; Intestinal trefoil factor
氨基酸序列

YQGLATNLCEVPPKDRVDCGYPEITSEQCVNRGCCFDSSIHGVPWCFKPLQDTECRF

分子量

9.2 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

TFF3 Protein, Canine (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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