TGF beta 2/TGFB2 蛋白, Human (HEK293, Avi)

In the TGF-beta-2 pathway, TGF beta 2/TGFB2 protein acts as a precursor for Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2), regulating their formation. Crucially, TGF beta 2/TGFB2 maintains TGF-beta-2 in a latent state within the extracellular matrix, forming non-covalent associations with TGF-beta-2. Interactions with 'milieu molecules' like LTBP1 and LRRC32/GARP finely tune TGF-beta-2 activation, playing a pivotal role in controlling this critical signaling pathway. TGF beta 2/TGFB2 Protein, Human (HEK293, Avi) is the recombinant human-derived TGF beta 2/TGFB2 protein, expressed by HEK293, with C-Avi labeled tag. The total length of TGF beta 2/TGFB2 Protein, Human (HEK293, Avi) is 112 a.a., with molecular weight of 14.53 kDa.

作为 TGF-β-2 信号通路的核心参与者，TGF-β2/TGFB2 蛋白承担潜伏相关肽 (LAP) 和转化生长因子 β-2 (TGF-β- 2）链条。这些链共同分别形成 TGF-β-2 的调节亚基和活性亚基。值得注意的是，TGF-β2/TGFB2 对于在细胞外基质内储存期间维持 TGF-β-2 链处于潜在状态至关重要。该蛋白与 TGF-β-2 进行非共价结合，通过与“环境分子”（包括 LTBP1 和 LRRC32/GARP）相互作用来复杂地调节其激活。这些相互作用在控制 TGF-β-2 的激活中发挥着关键作用，有助于这一关键信号通路的精细调节。

Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 2 μg/mL (100 μl/well) on the streptavidin precoated plate (5 μg/mL). Dose response curve for Human TGF-beta RII, mFc Tag with the EC₅₀ of 0.75 μg/mL determined by ELISA.

Human

HEK293

C-Avi

P61812 (A303-S414)

7042  [NCBI]

14.53 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of 4mM HCl. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in 4mM HCl, pH 3.0.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.