1. 重组蛋白
  2. CD Antigens
  3. Macrophage CD Proteins Stem Cell CD Proteins Endothelial cell CD Proteins
  4. Thy-1/CD90
  5. Thy1/CD90 蛋白, Cynomolgus (HEK293, His)

Thy1/CD90 蛋白, Cynomolgus (HEK293, His)

目录号: HY-P76105
COA 产品使用指南

Thy1/CD90 蛋白是免疫球蛋白家族的成员。它在许多细胞类型的细胞粘附和细胞通讯中发挥作用,特别是在免疫和神经系统的细胞中。Thy1/CD90 蛋白 具有多种免疫功能,同时还参与轴突再生、凋亡、粘附、迁移、癌症和纤维化中细胞-细胞和细胞-基质相互作用的调节。Thy1/CD90 蛋白, Cynomolgus (HEK293, His) 是重组的 Thy1/CD90 蛋白,由 HEK293 表达,带有 C-His 标签。Thy1/CD90 蛋白, Cynomolgus (HEK293, His) 全长 110 个氨基酸,分子量为 27-32 kDa。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

Thy1/CD90 Protein is a member of the immunoglobulin family. It plays a role in cell adhesion and cell communication in many cell types, especially in cells of the immune and nervous systems.Thy1/CD90 protein has multiple immune functions and also participates in the regulation of cell-cell and cell-matrix interactions in axonal regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. Thy1/CD90 Protein, Cynomolgus (HEK293, His) is the recombinant cynomolgus-derived Thy1/CD90 protein, expressed by HEK293 , with C-His labeled tag. The total length of Thy1/CD90 Protein, Cynomolgus (HEK293, His) is 110 a.a., with molecular weight of 27-32 kDa.

研究背景

Thy1/CD90 Protein 具有多种免疫功能。大多数通过与整合素相互作用介导[1][2] Thy1/CD90 蛋白是一种活化决定簇。使用 MAb 结合和交联 T 细胞表面的 Thy1/CD90 Protein 可能导致 T 细胞活化,其方式类似于植物凝集素,导致多克隆诱导白细胞介素 2(IL-2) 的产生、IL-2R 表达和增殖[3]

生物活性

Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus CD90 is immobilized at 5.00 µg/mL (100 µL/well), recombinant Human Galectin-1 binds with an ED50 of 0.4373 μg/mL.

种属

Cynomolgus

表达系统

HEK293

标签

C-His

蛋白编号

A0A2K5U4H6 (Q20-K129)

基因 ID

102139037  [NCBI]

蛋白结构
N-term
Thy1 (Q20-K129)
Accession # A0A2K5U4H6
His
C-term
同用名
Thy-1 membrane glycoprotein; Thy-1 antigen; CD90; Thy-1
氨基酸序列

QKVTSLTACLVDQSLRLDCRHENTTSSPIQYEFSLTRETKKHVLFGTVGVPEHTYRSRTNFTSKYNMKVLYLSAFTSKDEGTYTCALHHSGHSPPISSQNVTVLRDKLVK

分子量

Approximately 27 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Thy1/CD90 Protein, Cynomolgus (HEK293, His)
目录号:
HY-P76105
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