1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TNF Superfamily Neurotrophic Factors
  4. TNF Superfamily Ligands
  5. TNF-alpha
  6. TNF-alpha/TNFSF2 蛋白, Human

TNF-alpha/TNFSF2 蛋白, Human

目录号: HY-P7058
COA 产品使用指南

肿瘤坏死因子 α (TNF alpha) 是一种有效的促炎细胞因子。TNF alpha 与其受体结合,主要是 TNFR1 和 TNFR2,然后传递信号以实现炎症和细胞死亡等生物学功能。TNF alpha 通过 TNFR2 受体激活 NF-κB 通路促进癌细胞生长、侵袭和转移,但抗 TNF-α 抗体能显着抑制结肠炎相关癌症 (CAC) 小鼠的肿瘤发展。TNF alpha 作为一种原神经原因子可激活 SAPK/JNK 通路,并能够促进神经元替代和脑修复以应对脑损伤。TNF-alpha/TNFSF2 蛋白, Human 是一种由大肠杆菌表达的重组蛋白,由 157 个 (V77-L233) 氨基酸组成。

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

  • 参考文献

描述

Tumour Necrosis Factor alpha (TNF alpha) is a potent pro-inflammatory cytokine[1]. TNF alpha binds to its receptors, mainly TNFR1 and TNFR2, and then transmits molecular signals for biological functions such as inflammation and cell death[2]. TNF alpha stimulates NF-κB pathway via TNFR2 promotes cancer growth, invasion, and metastasis. Anti-TNF-α MAb significantly suppresses the tumor development in colitis-associated cancer (CAC) mouse[3]. TNF alpha as a proneurogenic factor activates the SAPK/JNK Pathway and can facilitate neuronal replacement and brain repair in response to brain injury[4]. TNF-alpha/TNFSF2 Protein, Human is a recombinant protein consisting of 157 amino acids (V77-L233) and is produced in E. coli.

研究背景

TNF α 由巨噬细胞、单核细胞、中性粒细胞、T 细胞和 NK 细胞等多种细胞产生[2]
人 TNF α 蛋白的氨基酸序列同源性较低小鼠、大鼠、牛、食蟹猴之间的 TNF α 蛋白。而人TNFα与食蟹猴TNFα蛋白的氨基酸序列一致性为94.85%,小鼠TNFα与大鼠TNFα蛋白的氨基酸序列一致性为94.47%。
TNFα以两种形式存在;II 型跨膜蛋白 (tmTNF-α) 和成熟可溶性蛋白 (sTNF-α)。TNF-α与其受体(主要是TNFR1和TNFR2)结合,然后传递分子信号以实现炎症和细胞死亡等生物学功能。sTNF-α 和 tmTNF-α 均激活 TNFR1,并处理与 TNFR1 相关死亡结构域 (TRADD) 接头蛋白相互作用的死亡结构域 (DD)。TNFR2信号通路主要由tmTNF-α激活。TNFR1 信号传导往往具有促炎和凋亡作用。TNFR2 会导致 NF-κB 和 MAPK 以及 AKT 激活,TNFR2 激活与组织再生、细胞增殖和细胞存活以及宿主防御和炎症等稳态生物活性相关[1]
TNF-α 对正常免疫反应至关重要,异常分泌 TNF-α 激活滑膜成纤维细胞、角质形成细胞、破骨细胞,诱发类风湿性关节炎、炎症性肠病、银屑病关节炎 (PsA) 和非感染性葡萄膜炎 (NIU)[3]。TNF α 独立于 Pgp 外排活性正向调节内源 TNF-α 表达水平,诱导 IHF 细胞增殖[4]。组织中的 TNF α 可能促进癌症生长、侵袭和转移。此外,TNF α 通过 TNFR2 刺激 NF-κB 通路,而抗 TNF-α MAb 显着抑制结肠炎相关癌 (CAC) 小鼠的肿瘤发展[5]。TNF α 作为一种原神经生成因子,可激活 SAPK/JNK 通路,并可促进脑损伤后的神经元替换和脑修复[6]

体外研究
(In Vitro)

TNF alpha (重组人源蛋白) (0, 10, 15, 20, 30, 50 ng/mL; 24, 48 h) 在 30 ng/mL 浓度,48 小时后降低 KB-C1 细胞活力,减少 pro-caspase-3,裂解的 caspase -3 在 KB-C1 和 KB-3-1 细胞中的表达[6].
TNF alpha (重组人源蛋白) (10, 15 ng/mL; 24 h) 显着增加 KB-3-1 细胞中 Pgp (ABCB1) 的 mRNA 水平,促进总 Pgp 蛋白水平的表达降低,轻微降低 KB-C1 细胞表面的的 Pgp 蛋白水平,并可能通过 ERK 激活刺激 IHF 细胞增殖[6]

体内研究
(In Vivo)

TNF alpha (重组人源蛋白) (100 µg; 后背植入锇泵; 每日注入,共 7 天) 在 SCID-HuRAg 小鼠中,显着增加炎症细胞的数量,并且滑膜炎症的程度显着加剧[7]

生物活性

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is ≤178.1 pg/mL.

  • Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 62.25 pg/mL, corresponding to a specific activity is 1.61×107units/mg.
种属

Human

表达系统

E. coli

标签

Tag Free

蛋白编号

P01375 (V77-L233)

基因 ID
蛋白结构
N-term
TGF-α (V77-L233)
Accession # P01375
C-term
中文名
重组人 TNF-alpha/TNFSF10 蛋白
同用名
rHuTNF-α/TNFSF2; TNF-alpha; Cachectin; DIF; TNFA; Differentiation-inducing factor; TNFα
氨基酸序列

VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

分子量

approximately 16-18 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized after extensive dialysis against PBS or 20 mM PB, 6% Sucrose, 4% Mannitol, 0.05% Tween 80, pH 6.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0 or PBS, pH 7.4, 8% trehalose, 8% mannitol and 0.02% Tween80.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
TNF-alpha/TNFSF2 Protein, Human
目录号:
HY-P7058
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