TRADD 蛋白, Human (GST)

TRADD protein acts as an adapter for TNFRSF1A/TNFR1, mediating its interaction with FADD. TRADD induces TNF-induced responses: apoptosis and NF-kappa-B activation. The nuclear form of TRADD functions as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A. Stimulation of TNFRSF1A forms plasma membrane-bound complex I, promoting cell survival. Subsequently, TRADD dissociates to form cytoplasmic complex II, inducing apoptosis. TRADD interacts with various proteins in these complexes, emphasizing its multifaceted role in cellular processes. TRADD Protein, Human (GST) is the recombinant human-derived TRADD protein, expressed by E. coli, with N-GST labeled tag. The total length of TRADD Protein, Human (GST) is 312 a.a., with molecular weight of 61.2 kDa.

TRADD 蛋白作为 TNFRSF1A/TNFR1 的衔接分子，与激活的 TNFRSF1A/TNFR1 的细胞质结构域特异性结合，介导其与 FADD 的相互作用。TRADD 的过度表达会诱导 TNF 诱导的两种主要反应：细胞凋亡和 NF-κ-B 的激活。TRADD 的核形式通过与 TRIP12 相互作用，阻止 CDKN2A 的同种型 p19ARF/ARF 泛素化和降解，从而破坏 TRIP12 与 CDKN2A 的同种型 p19ARF/ARF 之间的相互作用，从而起到肿瘤抑制剂的作用。TNF-α 受体 TNFRSF1A 的刺激会导致两种不同信号复合物的形成。质膜结合复合物 I 由 TNFRSF1A、TRADD、RIPK1、TRAF2 和 BIRC2/c-IAP1 或 BIRC3 组成，与 CHUCK/IKK-alpha、IKBKB/IKK-beta 和 IKBKG/IKK-gamma 相互作用，促进细胞存活。随后，TRADD、RIPK1和TRAF2与TNFRSF1A解离，与FADD和caspase CASP8形成细胞质复合物II，促进细胞凋亡。TRADD 还与这些复合物中的各种蛋白质相互作用，包括 TRPC4AP、UXT1、DAB2IP、SQSTM1、TRIP12、HIPK2、KRT14、KRT18、KRT16、KRT17、FADD 和 TOMM70，突出了其在不同细胞过程中的多方面作用。

Human

E. coli

N-GST

Q15628 (M1-A312)

8717

61.2kDa

Greater than 90 % as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.