TRAIL/TNFSF10 蛋白, Mouse (His,Solution)

TRAIL Protein (TNFSF10), a member of the TNF superfamily, is a type II transmembrane protein. TRAIL Protein mainly interacts with TRAIL-R1 and TRAIL-R2, and induces apoptosis in tumor or infected cells. TRAIL Protein also binds with DR4, DR5, and OPG. TRAIL Protein can recruit FADD and further recruit and activates caspase-8 after binding to DR4 or DR5. Besides, TRAIL may also trigger nonapoptotic signaling through activating pro-inflammatory pathways^[1][2]. TRAIL protein is mainly expressed on surface of immune cells, such as cytotoxic T cells and natural killer (NK) cell^[1]. TRAIL/TNFSF10 Protein, Mouse (His) is a recombinant mouse TRAIL (P118-N291) with N-terminal His tag, which is expressed in E.coli.

TRAIL 蛋白（TNFSF10）是 TNF 超家族的成员，是一种 II 型跨膜蛋白。TRAIL蛋白在多种组织中表达，特别是在脾、肺和前列腺中。TRAIL蛋白主要表达于免疫细胞表面，如细胞毒性T细胞和自然杀伤（NK）细胞。NK 和 T 细胞上的 TRAIL 蛋白对于控制病毒感染和肿瘤免疫监视至关重要^[1][2]。
小鼠 TRAIL 由细胞质结构域 (M1 -R17)、螺旋结构域 (M18-T38) 和胞外结构域 (Y39-N291)。小鼠 TRAIL 蛋白与人类具有 < 70% 的共同氨基酸同一性。小鼠 TRAIL 蛋白与大鼠有 86.94% 的共同氨基酸同一性。
TRAIL蛋白主要与两种激动性TRAIL受体（TRAIL-R1和TRAIL-R2）相互作用，诱导肿瘤或感染细胞凋亡。TRAIL 蛋白还与 DR4、DR5 和 OPG 结合。当与 DR4 或 DR5 结合时，TRAIL 蛋白可以招募 FADD，并进一步招募并激活 caspase-8。此外，TRAIL 还可能通过激活促炎通路（如 NF-κB、PI3K/Akt 和 MAPK 通路）来触发非凋亡信号传导^[1][2]。
TRAIL 以不依赖 p53 的方式诱导肿瘤细胞凋亡。基于 TRAIL 的疗法具有很高的抗肿瘤潜力^[3]。

TRAIL (mouse, -3 ng/mL, 6 d) results in the release of substantial amounts of IL-13 and IL-5 from PBLN cells isolated from mice^[4].

TRAIL (mouse, 1 μg, i.n.) induces the accumulation of both mDCs (MHCII^low) and CD4+ T cells in Wild-type mice^[4].

Mouse

E. coli

N-His

P50592 (P118-N291)

22035  [NCBI]

PRGGRPQKVAAHITGITRRSNSALIPISKDGKTLGQKIESWESSRKGHSFLNHVLFRNGELVIEQEGLYYIYSQTYFRFQEAEDASKMVSKDKVRTKQLVQYIYKYTSYPDPIVLMKSARNSCWSRDAEYGLYSIYQGGLFELKKNDRIFVSVTNEHLMDLDQEASFFGAFLIN

Approximately 22.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 300 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Cardoso Alves L, et al. The multifaceted role of TRAIL signaling in cancer and immunity. FEBS J. 2021 Oct;288(19):5530-5554.  [Content Brief]

  [2]. Pitti RM, et al. Induction of apoptosis by Apo-2 ligand, a new member of the tumor necrosis factor cytokine family. J Biol Chem. 1996 May 31;271(22):12687-90.  [Content Brief]

  [3]. Zhong HH, et al. TRAIL-based gene delivery and therapeutic strategies. Acta Pharmacol Sin. 2019 Nov;40(11):1373-1385.  [Content Brief]

  [4]. Wang S, et al. TRAIL and apoptosis induction by TNF-family death receptors. Oncogene. 2003 Nov 24;22(53):8628-33.

  [5]. Snajdauf M, et al. The TRAIL in the Treatment of Human Cancer: An Update on Clinical Trials. Front Mol Biosci. 2021 Mar 10;8:628332.  [Content Brief]

  [6]. Almasan A, et al. Apo2L/TRAIL: apoptosis signaling, biology, and potential for cancer therapy. Cytokine Growth Factor Rev. 2003 Jun-Aug;14(3-4):337-48.  [Content Brief]

  [7]. Weckmann M, et al. Critical link between TRAIL and CCL20 for the activation of TH2 cells and the expression of allergic airway disease. Nat Med. 2007 Nov;13(11):1308-15.  [Content Brief]