TROY/TNFRSF19 蛋白, Human (HEK293, His)

TROY Protein also known as TAJ, TNFRSF19, a type I transmembrane receptor, is a member of the TNF receptor superfamily. TROY Protein is involved in embryonic development and the development of skin and hair follicles^[1]. TROY Protein inhibits cell proliferation in vitro and shows antitumor activity in xenografted models^[2]. TROY/TNFRSF19 Protein, Human (HEK293, His) is a recombinant protein with a C-Terminal His label, It consists of 170 amino acids (M1-L170) and is produced in HEK293 cells.

TROY 蛋白在胚胎中以及出生后显示出独特的组织分布；在胚胎中，TROY 只在上皮细胞中表达，包括神经上皮、皮肤、细支气管上皮、结膜等，而出生后，TROY 蛋白在 Edar 等毛囊以及神经元中强烈表达^[1]。TROY 蛋白在多种侵袭性癌症中表达，包括结直肠癌、肺癌、黑色素瘤、鼻咽癌、前列腺癌和 GBM^[3]。
人 TROY 蛋白的氨基酸序列具有低与小鼠 TROY 蛋白具有同源性。
TROY 蛋白是一种生长促进信号分子，还通过与 RKIP 相互作用来调节 NF-κB 通路^[2]。此外，TROY 表达增加会促进 STAT3 磷酸化和依赖于 JAK1^[3] 的 STAT3 转录活性。TROY 与 LGR5 相互作用并抑制 Wnt 信号传导^[4]。
TROY 的敲低可抑制胶质瘤细胞的生长并诱导 U87 细胞的细胞周期停滞在 G1-S 期，效果显着降低 NF-kB 荧光素酶活性^[2]。TROY 表达增加会增加 JAK1 磷酸化，而沉默 TROY 表达会抑制 GBM 细胞侵袭，并增加胶质母细胞瘤中对替莫唑胺的敏感性^[3]。

Human

HEK293

C-His

Q9NS68-2 (E30-L170)

55504  [NCBI]

ESGDCRQQEFRDRSGNCVPCNQCGPGMELSKECGFGYGEDAQCVTCRLHRFKEDWGFQKCKPCLDCAVVNRFQKANCSATSDAICGDCLPGFYRKTKLVGFQDMECVPCGDPPPPYEPHCASKVNLVKIASTASSPRDTAL

25-40kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Kojima T, et al. TROY, a newly identified member of the tumor necrosis factor receptor superfamily, exhibits a homology with Edar and is expressed in embryonic skin and hair follicles. J Biol Chem. 2000 Jul 7;275(27):20742-7.  [Content Brief]

  [2]. Liu X, et al. TROY interacts with RKIP to promote glioma development. Oncogene. 2019 Feb;38(9):1544-1559.  [Content Brief]

  [3]. Ding Z, et al. TROY signals through JAK1-STAT3 to promote glioblastoma cell migration and resistance. Neoplasia. 2020 Sep;22(9):352-364.  [Content Brief]

  [4]. Fafilek B, et al. Troy, a tumor necrosis factor receptor family member, interacts with lgr5 to inhibit wnt signaling in intestinal stem cells. Gastroenterology. 2013 Feb;144(2):381-391.  [Content Brief]