1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. UbcH6/UBE2E1 蛋白, Human (Sf9, His, Strep)

UbcH6/UBE2E1 蛋白, Human (Sf9, His, Strep)

目录号: HY-P701347
产品使用指南

UbcH6/UBE2E1 蛋白是一种重要的泛素化成分,充当 E2 泛素结合酶,接受 E1 复合物中的泛素并催化其与蛋白质的共价附着。它用途广泛,还可促进 ISG15 的共价连接,在各种泛素样修饰中发挥作用。UbcH6/UBE2E1 蛋白, Human (Sf9, His, Strep) 是重组的 UbcH6/UBE2E1 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-8*His 标签。UbcH6/UBE2E1 蛋白, Human (Sf9, His, Strep) 全长 192 个氨基酸。

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描述

The UbcH6/UBE2E1 protein is an important ubiquitination component that acts as an E2 ubiquitin-conjugating enzyme, accepting ubiquitin in the E1 complex and catalyzing its covalent attachment to proteins. It is versatile and also facilitates covalent attachment of ISG15, playing a role in a variety of ubiquitin-like modifications. UbcH6/UBE2E1 Protein, Human (Sf9, His, Strep) is the recombinant human-derived UbcH6/UBE2E1 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UbcH6/UBE2E1 Protein, Human (Sf9, His, Strep) is 192 a.a., .

研究背景

UbcH6/UBE2E1 是泛素化机制的关键组成部分,通过接受 E1 复合物中的泛素并催化其与其他蛋白质的共价连接,发挥 E2 泛素结合酶的作用。这种酶用途广泛,还可以促进 ISG15 与其他蛋白质的共价连接,突出其在多种泛素样修饰中的作用。除了传统的泛素化之外,UbcH6/UBE2E1 在选择性降解短寿命和异常蛋白质方面发挥着至关重要的作用,有助于细胞质量控制机制。此外,体外研究证明其能够催化“Lys-48”连接的多聚泛素化,强调其参与蛋白质稳定性和周转的调节。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-8*His

蛋白编号

P51965 (S2-T193)

基因 ID

7324

蛋白结构
N-term
8*His-Strep
UbcH6 (S2-T193)
Accession # P51965
C-term
同用名
UBE2E1; Ubiquitin-conjugating enzyme E2 E1; (E3-independent) E2 ubiquitin-conjugating enzyme E1; E2 ubiquitin-conjugating enzyme E1; UbcH6; Ubiquitin carrier protein E1; Ubiquitin-protein ligase E1
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UbcH6/UBE2E1 Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UbcH6/UBE2E1 Protein, Human (Sf9, His, Strep)
目录号:
HY-P701347
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