UbcH7/UBE2L3 蛋白, Human

UbcH7/UBE2L3 is a unique ubiquitin-conjugating E2 enzyme that cooperates with HECT-type and RBR family E3 ligases and lacks intrinsic E3-independent reactivity with lysine. It uniquely works with RBR family E3 enzymes such as PRKN, RNF31 and ARIH1, acting like a RING-HECT hybrid. UbcH7/UBE2L3 Protein, Human is the recombinant human-derived UbcH7/UBE2L3 protein, expressed by E. coli , with tag free. The total length of UbcH7/UBE2L3 Protein, Human is 154 a.a., with molecular weight of ~18 kDa.

UbcH7/UBE2L3 是一种泛素结合酶 E2，与 HECT 型和 RBR 家族 E3 泛素蛋白连接酶合作表现出特异性。值得注意的是，其独特的特征是不存在与赖氨酸的内在 E3 独立反应性，使其与大多数含有 RING 的 E3 泛素蛋白连接酶不相容。然而，它表现出对 RBR 家族 E3 酶（如 PRKN、RNF31 和 ARIH1）的活性，其功能类似于 RING-HECT 杂交体。UbcH7 作用于 E1 复合物下游，催化泛素与靶蛋白的共价连接，并在体外促进“Lys-11”连接的多泛素化。它参与选择性降解短寿命和异常蛋白质，凸显了它在细胞质量控制中的作用。此外，S 期期间的下调表明对细胞周期进程的贡献，而其对核激素受体转录活性的影响和在骨髓生成中的潜在作用进一步强调了其多方面的功能。

Recombinant Human UbcH7/UBE2L3 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins.

Human

E. coli

Tag Free

P68036 (M1-D154)

7332  [NCBI]

MAASRRLMKELEEIRKCGMKNFRNIQVDEANLLTWQGLIVPDNPPYDKGAFRIEINFPAEYPFKPPKITFKTKIYHPNIDEKGQVCLPVISAENWKPATKTDQVIQSLIALVNDPQPEHPLRADLAEEYSKDRKKFCKNAEEFTKKYGEKRPVD

Approximately 18 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 20 mM Hepes, 50 mM Nacl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.