1. 重组蛋白
  2. Enzymes & Regulators
  3. Ligases (EC 6)
  4. UBE1L2/UBA6 蛋白, Human (Sf9, His, Strep)

UBE1L2/UBA6 蛋白, Human (Sf9, His, Strep)

目录号: HY-P701353
产品使用指南

泛素 E1 酶通过用 ATP 将泛素腺苷酸化并与 E1 酶形成硫酯键来启动泛素化。它对泛素表现出特异性,并排除泛素样肽。UBE1L2/UBA6 蛋白, Human (Sf9, His, Strep) 是重组的 UBE1L2/UBA6 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-8*His 标签。UBE1L2/UBA6 蛋白, Human (Sf9, His, Strep) 全长 1051 个氨基酸。

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描述

The ubiquitin E1 enzyme initiates ubiquitination by adenylating ubiquitin with ATP and forming a thioester bond with the E1 enzyme. It exhibits specificity for ubiquitin and excludes ubiquitin-like peptides. UBE1L2/UBA6 Protein, Human (Sf9, His, Strep) is the recombinant human-derived UBE1L2/UBA6 protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UBE1L2/UBA6 Protein, Human (Sf9, His, Strep) is 1051 a.a., .

研究背景

泛素 E1 酶通过两步机制激活泛素来启动泛素化过程。它首先用 ATP 将泛素的 C 端甘氨酸残基腺苷酸化,然后在该甘氨酸残基和 E1 酶中的半胱氨酸残基之间形成硫酯键,在此过程中释放出游离的 AMP。值得注意的是,它表现出对泛素的特异性,将泛素样肽排除在该激活过程之外。与 UBE1 相比,该酶对充电底物 E2 酶具有选择性,不包括细胞周期 E2,例如 CDC34。它在胚胎发育中的作用强调了它的不可或缺性,并且它对于 UBD/FAT10 的结合至关重要。该酶的亚型 2 可能对泛素系统产生重大影响,可能影响精子发生和男性生育能力。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-8*His

蛋白编号

A0AVT1 (E2-D1052)

基因 ID

55236

蛋白结构
N-term
8*His-Strep
UBE1L2 (E2-D1052)
Accession # A0AVT1
C-term
同用名
UBA6; Ubiquitin-like modifier-activating enzyme 6; Ubiquitin-activating enzyme 6; Monocyte protein 4; MOP-4; Ubiquitin-activating enzyme E1-like protein 2; E1-L2
纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UBE1L2/UBA6 Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
UBE1L2/UBA6 Protein, Human (Sf9, His, Strep)
目录号:
HY-P701353
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