UBE2A 蛋白, Human (GST-His)

UBE2A cooperates with the E3 enzyme BRE1 to promote the attachment of ubiquitin to proteins, specifically histone H2B at "Lys-120", forming H2BK120ub1. This modification serves as a tag for transcriptional activation, RNA polymerase II elongation, telomeric silencing, and is critical for the formation of H3K4me and H3K79me. UBE2A Protein, Human (GST-His) is the recombinant human-derived UBE2A protein, expressed by E. coli , with N-6*His, N-GST labeled tag. The total length of UBE2A Protein, Human (GST-His) is 152 a.a., with molecular weight of ~52.0 kDa.

UBE2A 作为泛素-蛋白酶体系统的一个组成部分，充当泛素从 E1 复合物转移到靶蛋白的导管，催化它们的共价附着。UBE2A 与 E3 酶 BRE1（RNF20 和/或 RNF40）合作，通过协调组蛋白 H2B 在“Lys-120”处的单泛素化，从而在转录调节中发挥关键作用，从而形成 H2BK120ub1。这种修饰是表观遗传转录激活、RNA 聚合酶 II 延伸、端粒沉默的独特标记，对于 H3K4me 和 H3K79me 的后续形成至关重要。除了在组蛋白泛素化中发挥作用外，UBE2A 还表现出体外催化活性，介导“Lys-11”和“Lys-48”连接的多泛素化。此外，UBE2A 对于紫外线损伤 DNA 的复制后修复是不可或缺的，这凸显了它在基本细胞过程中的多方面参与。

E2-ubiquitin conjugation was detected by whether the DTT-sensitive thioester linkage can form or not.

Human

E. coli

N-6*His;N-GST

P49459-1 (M1-C152)

7319  [NCBI]

MSTPARRRLMRDFKRLQEDPPAGVSGAPSENNIMVWNAVIFGPEGTPFEDGTFKLTIEFTEEYPNKPPTVRFVSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTSIQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSWRDC

Approximately 52.0 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, 2 mM DTT, 10% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.