UBE2G1 蛋白, Human

UBE2G1 Protein, a crucial ubiquitin-proteasome system player, acts as an E2 ubiquitin-conjugating enzyme, facilitating ubiquitin attachment to diverse protein substrates. In vitro, UBE2G1 demonstrates catalytic competence in 'Lys-48' and 'Lys-63'-linked polyubiquitination reactions. This multifaceted enzyme is implicated in potential roles, including the degradation of muscle-specific proteins, emphasizing its involvement in cellular processes related to protein turnover. Additionally, UBE2G1 mediates the polyubiquitination of CYP3A4, a cytochrome P450 enzyme, underscoring its significance in regulating specific cellular pathways. UBE2G1 Protein, Human is the recombinant human-derived UBE2G1 protein, expressed by E. coli, with tag free. The total length of UBE2G1 Protein, Human is 170 a.a., with molecular weight of ~19.5 kDa.

UBE2G1 是泛素-蛋白酶体系统中的重要参与者，作为 E2 泛素结合酶，促进泛素与多种蛋白质底物的共价附着。在体外，UBE2G1 通过催化“Lys-48”和“Lys-63”连接的多泛素化反应展示其催化能力。这种多方面的酶涉及潜在的作用，包括肌肉特异性蛋白质的降解，突出显示它参与与蛋白质周转相关的细胞过程。此外，UBE2G1 通过介导 CYP3A4（一种参与各种底物代谢的细胞色素 P450 酶）的多泛素化来证明其功能相关性，强调了其在调节特定细胞途径中的重要性。

Under ATP and E1 conditions, E2 covalently binds with ubiquitin molecules to form thioester complexes E2~Ub.

Human

E. coli

Tag Free

P62253 (M1-E170)

7326  [NCBI]

MTELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIWHPNVDKNGDVCISILHEPGEDKYGYEKPEERWLPIHTVETIMISVISMLADPNGDSPANVDAAKEWREDRNGEFKRKVARCVRKSQETAFE

Approximately 19.5 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, 10% Glycerol, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.