UBE2I 蛋白, Human (GST)

UBE2I Protein, a key participant in protein sumoylation, accepts ubiquitin-like proteins SUMO1, SUMO2, SUMO3, SUMO4, and SUMO1P1/SUMO5 from the UBLE1A-UBLE1B E1 complex. Teaming up with E3 ligases like RANBP2, CBX4, and ZNF451, UBE2I catalyzes the covalent attachment of these SUMO proteins to target proteins, including critical substrates FOXL2 and KAT5. Its enzymatic activity extends to forming poly-SUMO chains, influencing diverse cellular processes and playing a pivotal role in specific protein modifications, such as sumoylation of p53/TP53 at 'Lys-386' and ERCC6. UBE2I Protein, Human (GST) is the recombinant human-derived UBE2I protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2I Protein, Human (GST) is 158 a.a., with molecular weight of ~40.0 kDa.

UBE2I 是蛋白质苏酰化过程中的关键参与者，它通过接受 UBLE1A-UBLE1B E1 复合物中的泛素样蛋白 SUMO1、SUMO2、SUMO3、SUMO4 和 SUMO1P1/SUMO5 展现出多功能性。UBE2I 与各种 E3 连接酶（例如 RANBP2、CBX4 和 ZNF451）协作，催化这些 SUMO 蛋白与靶蛋白的共价连接。这种酶活性延伸至聚 SUMO 链的形成，强调了其在多种细胞过程中的作用。UBE2I 对于涉及 FOXL2 和 KAT5 等关键底物的苏酰化事件至关重要，有助于核结构和染色体分离。此外，UBE2I 介导的苏酰化在特定蛋白质修饰中发挥着关键作用，包括 p53/TP53 在“Lys-386”和 ERCC6 处的苏酰化，后者对于其转录偶联的核苷酸切除修复活性是不可或缺的。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-GST

P63279 (M1-S158)

7329  [NCBI]

MSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Approximately 40.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.2 μm filtered solution of 50 mM HEPES, 150 mM NaCl, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.