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  3. UBE2R1/CDC34 蛋白, Human (Sf9, His, Strep)

UBE2R1/CDC34 蛋白, Human (Sf9, His, Strep)

目录号: HY-P79448
产品使用指南

UBE2R1/CDC34 蛋白是重要的泛素-蛋白酶体系统组件,充当 E2 泛素结合酶,能够熟练地接受 E1 复合物中的泛素并催化其与多种蛋白质底物的共价附着。。在体外,UBE2R1 有效介导“Lys-48”连接的多聚泛素化。UBE2R1/CDC34 蛋白, Human (Sf9, His, Strep) 是重组的 UBE2R1/CDC34 蛋白,由 Sf9 insect cells 表达,带有 N-Strep, N-His 标签。UBE2R1/CDC34 蛋白, Human (Sf9, His, Strep) 全长 235 个氨基酸。

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描述

The UBE2R1/CDC34 protein is an important ubiquitin-proteasome system component that functions as an E2 ubiquitin-conjugating enzyme, capable of proficiently accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to a variety of protein substrates. . In vitro, UBE2R1 efficiently mediates “Lys-48”-linked polyubiquitination. UBE2R1/CDC34 Protein, Human (Sf9, His, Strep) is the recombinant human-derived UBE2R1/CDC34 protein, expressed by Sf9 insect cells , with N-Strep, N-His labeled tag. The total length of UBE2R1/CDC34 Protein, Human (Sf9, His, Strep) is 235 a.a., .

研究背景

UBE2R1 是泛素-蛋白酶体系统中的关键成分,作为 E2 泛素结合酶发挥作用,擅长从 E1 复合物中接受泛素并催化其与各种蛋白质底物的共价附着。在体外,UBE2R1 通过有效介导“Lys-48”连接的多泛素化反应展示了其催化能力。UBE2R1 与特定的 E3 连接酶复合物(例如 SCF(FBXW11) 和 SCF(SKP2))配合,在协调关键调节蛋白的泛素依赖性降解中发挥着至关重要的作用。值得注意的是,它参与 NFKBIA 的多泛素化,导致其随后的蛋白酶体降解,从而影响与细胞增殖相关的过程。UBE2R1 的参与范围扩展到细胞周期进程的调节,靶向 WEE1 激酶和 β-连环蛋白等蛋白质进行泛素化和降解。此外,它本身成为人类疱疹病毒 1 蛋白 ICP0 的靶标,与蛋白酶体建立动态相互作用,并有助于细胞蛋白稳态的复杂景观。

种属

Human

表达系统

Sf9 insect cells

标签

N-Strep;N-His

蛋白编号

P49427 (A2-S236)

基因 ID

997  [NCBI]

蛋白结构
N-term
8*His-Strep
UBE2R1 (A2-S236)
Accession # P49427
C-term
同用名
Ubiquitin-conjugating enzyme E2 R1; CDC34; (E3-independent) E2 ubiquitin-conjugating enzyme R1 (EC:2.3.2.24); E2 ubiquitin-conjugating enzyme R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; Ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protei
纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

UBE2R1/CDC34 Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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UBE2R1/CDC34 Protein, Human (Sf9, His, Strep)
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HY-P79448
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