UBE2S 蛋白, Human (GST)

UBE2S Protein, crucial in cellular ubiquitination, accepts ubiquitin from the E1 complex, catalyzing covalent attachment to various proteins. A key contributor to cell cycle regulation, UBE2S catalyzes 'Lys-11'-linked polyubiquitination, essential for anaphase promoting complex/cyclosome (APC/C) function. It plays a crucial role in mitotic progression by elongating 'Lys-11'-linked polyubiquitin chains initiated by UBE2C/UBCH10 on APC/C substrates, facilitating mitotic exit. Implicated in ubiquitination and degradation of VHL, UBE2S promotes polyubiquitination using all seven ubiquitin Lys residues in vitro, excluding 'Lys-48'-linked polyubiquitination, highlighting its versatility. UBE2S Protein, Human (GST) is the recombinant human-derived UBE2S protein, expressed by E. coli, with N-GST labeled tag. The total length of UBE2S Protein, Human (GST) is 222 a.a., with molecular weight of ~50.0 kDa.

UBE2S 是细胞泛素化过程中的关键参与者，它接受 E1 复合物中的泛素并催化其与各种蛋白质的共价附着。UBE2S 是细胞周期调节的关键贡献者，可催化“Lys-11”连接的多聚泛素化，特别是作为后期促进复合物/细胞周期体 (APC/C) 的重要因子。在这种情况下，UBE2S 通过特异性延长由 APC/C 底物上的 E2 酶 UBE2C/UBCH10 引发的“Lys-11”连接的多聚泛素链，在有丝分裂进展中发挥着至关重要的作用，导致蛋白酶体增强对这些底物的降解，并促进有丝分裂退出。此外，UBE2S 参与 VHL 的泛素化和随后的降解，从而导致 HIF1A 的积累。体外研究表明，UBE2S 能够利用所有七个泛素 Lys 残基（“Lys-48”连接的多泛素化除外）促进多泛素化，强调了其在泛素链形成中的多功能性。

Human

E. coli

N-GST

Q16763 (M1-L222)

27338  [NCBI]

MNSNVENLPPHIIRLVYKEVTTLTADPPDGIKVFPNEEDLTDLQVTIEGPEGTPYAGGLFRMKLLLGKDFPASPPKGYFLTKIFHPNVGANGEICVNVLKRDWTAELGIRHVLLTIKCLLIHPNPESALNEEAGRLLLENYEEYAARARLLTEIHGGAGGPSGRAEAGRALASGTEASSTDPGAPGGPGGAEGPMAKKHAGERDKKLAAKKKTDKKRALRRL

Approximately 50.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.