UBE2W 蛋白, Human (His)

UBE2W Protein, a versatile ubiquitin-conjugating enzyme, accepts ubiquitin from the E1 complex, catalyzing its covalent attachment to diverse substrates. Notably, UBE2W uniquely monoubiquitinates N-termini, including ATXN3, MAPT/TAU, POLR2H/RPB8, and STUB1/CHIP, displaying specificity for disordered N-termini. This monoubiquitination is crucial for degrading misfolded chaperone substrates, as UBE2W mediates STUB1/CHIP monoubiquitination, limiting ubiquitin chain length. Activated by UV irradiation, UBE2W collaborates with FANCL, catalyzing monoubiquitination of FANCD2 in the Fanconi anemia complex, a key step in the DNA damage response pathway. Additionally, UBE2W flexibly catalyzes 'Lys-11'-linked polyubiquitination in vitro. UBE2W Protein, Human (His) is the recombinant human-derived UBE2W protein, expressed by E. coli, with N-His labeled tag. The total length of UBE2W Protein, Human (His) is 151 a.a., with molecular weight of ~18 kDa.

UBE2W 是一种多功能泛素结合酶，通过接受 E1 复合物中的泛素并催化其与不同底物的共价附着，在细胞过程中发挥着至关重要的作用 (PubMed:20061386，PubMed:21229326)。值得注意的是，UBE2W 表现出独特的单泛素化各种底物 N 端的能力，包括 ATXN3、MAPT/TAU、POLR2H/RPB8 和 STUB1/CHIP，展示了其对无序 N 端的特异性 (PubMed:23560854、PubMed:23696636、公共医学：25436519）。这种单泛素化过程在错误折叠伴侣底物的降解中发挥着关键作用，因为 UBE2W 介导 STUB1/CHIP 的单泛素化，导致 ATXN3 的募集并限制附着在 STUB1/CHIP 底物上的泛素链长度 (PubMed:23696636，PubMed ：25436519）。此外，UBE2W，特别是通过紫外线照射激活，充当范可尼贫血复合体的特定 E2 泛素结合酶，与 E3 泛素蛋白连接酶 FANCL 合作，催化 FANCD2 的单泛素化，这是 DNA 损伤反应途径中的关键步骤 (PubMed ：19111657，PubMed：21229326）。除了其多功能的单泛素化能力外，UBE2W 还可以在体外催化“Lys-11”连接的多泛素化，证明其在泛素链形成方面的灵活性 (PubMed:25436519)。

Under ATP and E1 conditions, UBE2W binds with ubiquitin molecules to form thioester complexes UBE2W-Ub.

Human

E. coli

N-His

Q96B02 (M1-C151)

55284  [NCBI]

MASMQKRLQKELLALQNDPPPGMTLNEKSVQNSITQWIVDMEGAPGTLYEGEKFQLLFKFSSRYPFDSPQVMFTGENIPVHPHVYSNGHICLSILTEDWSPALSVQSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPKKTKWWYHDDTC

Approximately 18kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 100 mM Arg.0.1% Brij35, pH 8.5 or 50 mM Tris-HCL, 300 mM NaCl, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.