2. 重组蛋白
  3. Ubiquitin Related Proteins
  4. UE2NL 蛋白, Human (Sf9, His, Strep)

UE2NL 蛋白, Human (Sf9, His, Strep)

目录号: HY-P701497
产品使用指南

UE2NL 蛋白仅在附睾中表达，并调节该生殖组织中的分子过程。作为泛素结合酶家族的一部分，UE2NL 可能参与泛素介导的信号通路，影响附睾微环境中翻译后蛋白质的修饰。UE2NL 蛋白, Human (Sf9, His, Strep) 是重组的 UE2NL 蛋白，由 Sf9 insect cells 表达，带有 N-Strep, N-8*His 标签。UE2NL 蛋白, Human (Sf9, His, Strep) 全长 152 个氨基酸。

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描述

The UE2NL protein is expressed exclusively in the epididymis and regulates molecular processes in this reproductive tissue. As part of a family of ubiquitin-conjugating enzymes, UE2NL may be involved in ubiquitin-mediated signaling pathways affecting post-translational protein modifications in the epididymal microenvironment. UE2NL Protein, Human (Sf9, His, Strep) is the recombinant human-derived UE2NL protein, expressed by Sf9 insect cells , with N-Strep, N-8*His labeled tag. The total length of UE2NL Protein, Human (Sf9, His, Strep) is 152 a.a., .
研究背景

UE2NL 蛋白在附睾中以蛋白质水平特异性表达，表明其在调节该生殖组织内分子过程中的潜在作用。作为泛素结合酶家族的一员，UE2NL 可能参与泛素介导的信号通路，可能有助于附睾微环境中蛋白质的翻译后修饰。组织特异性表达强调了 UE2NL 在附睾功能中的重要性，强调其可能参与与附睾内生殖过程或精子成熟相关的分子事件。
种属

Human
表达系统

Sf9 insect cells
标签

N-Strep;N-8*His
蛋白编号

Q5JXB2 (A2-I153)
基因 ID

389898
蛋白结构
N-term
8*His-Strep
UE2NL (A2-I153)
Accession # Q5JXB2
C-term
同用名
UBE2NL; Putative ubiquitin-conjugating enzyme E2 N-like; Epididymis tissue protein Li 174
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

UE2NL Protein, Human (Sf9, His, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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