原位杂交技术 (ISH) 是一种用于检测组织或细胞中特定核酸序列的方法。通过将标记的核酸探针与样本中的目标 DNA 或 RNA 互相杂交，ISH 可在细胞或组织级别上定位特定基因或 RNA 分子的位置。该技术在研究基因表达、基因定位和细胞分化等领域中得到广泛应用。ISH 有多个变体，包括原位PCR、原位 RT-PCR 等，为深入了解生物学过程提供了强大的工具。

In situ hybridization (ISH) is a method used to detect specific nucleic acid sequences in tissues or cells. By hybridizing labeled nucleic acid probes to target DNA or RNA in a sample, ISH can locate the location of specific genes or RNA molecules at the cellular or tissue level. This technology is widely used in the study of gene expression, gene localization and cell differentiation. There are multiple variants of ISH, including in situ PCR, in situ RT-PCR, etc., which provide a powerful tool for in-depth understanding of biological processes.