Siglec-3/CD33 蛋白, Human (HEK293, Fc-Myc)

Siglec-3/CD33 protein is a sialic acid-binding lectin that mediates cell-cell interactions and maintains immune cell quiescence. It prefers α-2,3- and α-2,6-linked glycans with sialic acid. Siglec-3/CD33 Protein, Human (HEK293, Fc-Myc) is the recombinant human-derived Siglec-3/CD33 protein, expressed by HEK293 , with C-Myc, C-Fc labeled tag. The total length of Siglec-3/CD33 Protein, Human (HEK293, Fc-Myc) is 242 a.a., with molecular weight of ~92 kDa.

Siglec-3/CD33 蛋白是一种唾液酸结合免疫球蛋白样凝集素，在介导细胞间相互作用和维持免疫细胞处于静息状态方面发挥着至关重要的作用。它表现出对识别和结合 α-2,3- 和更热衷 α-2,6- 连接的唾液酸聚糖的偏好。在与 C1q 或唾液酸化糖蛋白等配体结合后，CD33 胞质尾部内的两个基于免疫受体酪氨酸的抑制基序 (ITIM) 会被 Src 样激酶（如 LCK）磷酸化。这些磷酸化的 ITIM 作为招募和激活蛋白酪氨酸磷酸酶 PTPN6/SHP-1 和 PTPN11/SHP-2 的对接位点，进而通过信号分子的去磷酸化调节下游通路。CD33 对单核细胞活化的抑制作用涉及磷酸肌醇 3-激酶/PI3K。在结构上，该蛋白通过二硫键形成同二聚体，并在磷酸化后与 PTPN6/SHP-1 和 PTPN11/SHP-2 相互作用。它还通过其 C 末端与 C1QA 结合，激活 CD33 抑制基序。

Measured by its binding ability in a functional ELISA. Immobilized CD33 at 2 μg/mL can bind Anti-CD33 rabbit monoclonal antibody and the EC₅₀ of human CD33 protein is 0.4375-0.6651 ng/mL.

Human

HEK293

C-Myc;C-Fc

P20138 (D18-H259)

945  [NCBI]

DPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISRDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH

Approximately 92 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm solution of PBS, 6% Trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.