1. 重组蛋白
  2. Fc Receptors Biotinylated Proteins
  3. FcRn
  4. FCGRT-B2M Heterodimer 蛋白, Human (Biotinylated, HEK293, His)

FCGRT-B2M Heterodimer 蛋白, Human (Biotinylated, HEK293, His)

目录号: HY-P701255
产品使用指南

FCGRT 蛋白是一种细胞表面受体,通过选择性摄取牛奶中的单体 IgG 来传递被动体液免疫。它在肠上皮上结合 IgG,并形成 FcRn-IgG 复合物,该复合物在上皮上转胞吞,将 IgG 释放到血液或组织中。FCGRT-B2M Heterodimer 蛋白, Human (Biotinylated, HEK293, His) 是重组的蛋白二聚体复合物,包含 FCGRT-B2M 异二聚体蛋白,由 HEK293 表达,带有 Strep, His 标签。FCGRT-B2M Heterodimer 蛋白, Human (Biotinylated, HEK293, His) ,分子量分别约为 ~33 kDa(FCGRT) & 13 kDa(B2M)。

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描述

The FCGRT protein is a cell surface receptor that conveys passive humoral immunity through the selective uptake of monomeric IgG in milk. It binds IgG at the intestinal epithelium and forms an FcRn-IgG complex that is transcytosed across the epithelium, releasing IgG into the blood or tissue. FCGRT-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His) is a recombinant protein dimer complex containing human-derived FCGRT-B2M Heterodimer protein, expressed by HEK293 , with Strep, His labeled tag. FCGRT-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His), has molecular weight of ~33 kDa(FCGRT) & 13 kDa(B2M), respectively.

研究背景

FCGRT 是一种重要的细胞表面受体,通过与单体免疫球蛋白 γ 的 Fc 区结合并选择性介导其从乳汁中的摄取,促进被动体液免疫从母亲转移到新生儿。该过程涉及 IgG 在肠上皮顶端表面结合,形成 FcRn-IgG 复合物,通过肠上皮转胞吞,将 IgG 释放到血液或组织液中。在婴儿期之后,FCGRT 通过回收 IgG 并延长其在循环中的半衰期,在有效的体液免疫中发挥着关键作用。从机制上讲,单体 IgG 与内皮细胞和造血细胞酸性内体中的 FCGRT 结合,促进 IgG 循环到细胞表面,随后将其释放到循环中。此外,FCGRT 调节白蛋白/ALB(另一种最丰富的循环蛋白)的稳态,进一步强调了其在免疫和蛋白质稳态中的重要作用。在微生物感染的情况下,FCGRT 充当各种埃可病毒的脱衣受体,包括埃可病毒 5、6、7、9、11、13、25 和 29。

种属

Human

表达系统

HEK293

标签

Strep;His

蛋白编号

P55899 (A24-S297)&P61769 (I21-M119)

基因 ID

2217&567

同用名
FcRn alpha chain; FCGRT; FCRN; FCGRT&B2M
分子量

Approximately 33 kDa(FCGRT)&13 kDa(B2M)

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

FCGRT-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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  • 重组蛋白稀释计算器

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
FCGRT-B2M Heterodimer Protein, Human (Biotinylated, HEK293, His)
目录号:
HY-P701255
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