Fumarate Hydratase/FH 蛋白, Human (His)

The fumarate hydratase/FH protein plays a key role in cellular processes as it catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, which is essential for energy production through the production of NADH . Fumarate Hydratase/FH Protein, Human (His) is the recombinant human-derived Fumarate Hydratase/FH protein, expressed by E. coli , with C-His labeled tag. The total length of Fumarate Hydratase/FH Protein, Human (His) is 466 a.a., with molecular weight of ~50 kDa.

富马酸水合酶 (FH) 是一种催化富马酸可逆转化为 L-苹果酸的酶。这种立体特异性互变在三羧酸（TCA）循环中发挥着关键作用，三羧酸循环是参与能量产生的中心代谢途径。具体来说，FH 促进富马酸水合为 L-苹果酸，有助于 TCA 循环中的关键过渡步骤。在不同物种中进行的实验表明，FH 的特定亚型可能在确定的途径中发挥作用，并且表现出对一个反应方向优于另一个反应方向的偏好。通过参与 TCA 循环，FH 确保以 NADH 形式有效产生能量，凸显其在细胞代谢中的重要作用。

Measured by its ability to transform 1 umole of Fumarate to L-malate per minute at pH 7.5 at 37°C. Specific activity is 22.89 U/mg.

Human

E. coli

C-His

P07954-1 (A45-K510)

2271  [NCBI]

ASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

Approximately 50 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris, 5 mM EDTA, 1 mM DTT, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.