1. 重组蛋白
  2. Cytokines and Growth Factors
  3. CSF & Receptors
  4. G-CSF
  5. G-CSF 蛋白, Human (HEK293)

G-CSF 蛋白, Human (HEK293)

目录号: HY-P73523
COA 产品使用指南

G-CSF 是一种关键细胞因子,通过控制粒细胞和单核巨噬细胞的产生、分化和功能来调节造血。这种单体蛋白专门促进粒细胞发育,在免疫系统调节和血细胞形成中发挥着至关重要的作用。G-CSF 蛋白, Human (HEK293) 是重组的 G-CSF 蛋白,由 HEK293 表达,不带标签。G-CSF 蛋白, Human (HEK293) 全长 175 个氨基酸,分子量约为 ~17.8 kDa。

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规格 价格 是否有货 数量
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2 μg ¥560 In-stock
5 μg ¥1230 In-stock
10 μg ¥2100 In-stock
50 μg ¥5880 In-stock
100 μg   询价  

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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

G-CSF, a pivotal cytokine, regulates hematopoiesis by controlling the production, differentiation, and function of granulocytes and monocytes-macrophages. This monomeric protein specifically promotes granulocyte development, playing a crucial role in immune system regulation and blood cell formation. G-CSF Protein, Human (HEK293) is the recombinant human-derived G-CSF protein, expressed by HEK293 , with tag free. The total length of G-CSF Protein, Human (HEK293) is 175 a.a., with molecular weight of ~17.8 kDa.

研究背景

粒细胞/巨噬细胞集落刺激因子 (G-CSF) 是一种对造血至关重要的细胞因子,控制着两种关键白细胞群的产生、分化和功能:粒细胞和单核细胞-巨噬细胞。作为一种单体蛋白,G-CSF 特异性诱导粒细胞发育,有助于调节免疫系统和血细胞形成。

生物活性

Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells and the ED50 is typically 0.04-0.2 ng/mL.

种属

Human

表达系统

HEK293

标签

Tag Free

蛋白编号

P09919-2/NP_757373.1 (A30-P204)

基因 ID
蛋白结构
N-term
G-CSF (A30-P204)
Accession # P09919-2
C-term
同用名
Granulocyte Colony-Stimulating Factor; G-CSF; CSF3; C17orf33
氨基酸序列

ATPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

分子量

Approximately 17.8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

G-CSF Protein, Human (HEK293) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
G-CSF Protein, Human (HEK293)
目录号:
HY-P73523
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