HPRG 蛋白, Human (HEK293, His)

HPRG protein is a multifunctional plasma glycoprotein with the ability to bind multiple ligands, including heme, heparin, thrombospondin, etc. HPRG regulates immune processes, angiogenesis, coagulation, and fibrinolysis through zinc-dependent binding to heparin and glycosaminoglycans. HPRG Protein, Human (HEK293, His) is the recombinant human-derived HPRG protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of HPRG Protein, Human (HEK293, His) is 507 a.a., with molecular weight of 75-80 kDa.

HPRG 蛋白是一种多功能血浆糖蛋白，具有多种配体结合能力，包括血红素、肝素、硫酸乙酰肝素、血小板反应蛋白、纤溶酶原和二价金属离子。它与肝素和肝素/糖胺聚糖表现出锌依赖性结合，并与肝、肺、肾和心脏内皮细胞表面的硫酸乙酰肝素相互作用。作为衔接蛋白，HPRG 参与多种过程的调节，例如免疫复合物和病原体清除、细胞趋化性、细胞粘附、血管生成、凝血和纤溶。HPRG 还通过增强硫酸乙酰肝素依赖性途径中的吞噬作用来介导坏死细胞的清除，并受到肝素和锌离子等特定配体的调节。此外，它与 IgG 亚类结合，影响其清除并抑制不溶性免疫复合物的形成。此外，HPRG 将纤溶酶原束缚在细胞表面，改变 T 细胞形态，并通过阻断 CD6 介导的抗血管生成作用来调节血管生成。HPRG 作为血管内皮生长因子 (VEGF) 信号通路的调节剂，抑制内皮细胞运动，在肿瘤血管生成和免疫监视中发挥作用，通过影响肿瘤相关巨噬细胞使肿瘤血管正常化并促进抗肿瘤免疫。

Human

HEK293

C-His;C-10*His

P04196/NP_000403.1 (V19-K525)

3273  [NCBI]

VSPTDCSAVEPEAEKALDLINKRRRDGYLFQLLRIADAHLDRVENTTVYYLVLDVQESDCSVLSRKYWNDCEPPDSRRPSEIVIGQCKVIATRHSHESQDLRVIDFNCTTSSVSSALANTKDSPVLIDFFEDTERYRKQANKALEKYKEENDDFASFRVDRIERVARVRGGEGTGYFVDFSVRNCPRHHFPRHPNVFGFCRADLFYDVEALDLESPKNLVINCEVFDPQEHENINGVPPHLGHPFHWGGHERSSTTKPPFKPHGSRDHHHPHKPHEHGPPPPPDERDHSHGPPLPQGPPPLLPMSCSSCQHATFGTNGAQRHSHNNNSSDLHPHKHHSHEQHPHGHHPHAHHPHEHDTHRQHPHGHHPHGHHPHGHHPHGHHPHGHHPHCHDFQDYGPCDPPPHNQGHCCHGHGPPPGHLRRRGPGKGPRPFHCRQIGSVYRLPPLRKGEVLPLPEANFPSFPLPHHKHPLKPDNQPFPQSVSESCPGKFKSGFPQVSMFFTHTFPK

75-80 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.