Matrix 蛋白 1/M1 蛋白, H3N2 (AFM71858, His)

Matrix protein 1/M1 Protein is pivotal in virus replication, spanning entry, uncoating, assembly, and budding. Binding to ribonucleocapsids inhibits viral transcription, and interaction with NEP aids nuclear export. M1 forms a shell on the inner virion membrane, binding the RNP. During entry, M1 dissociates from the RNP, allowing nuclear transport for transcription. M1 influences virion shape, determining infectivity, with filamentous virions crucial for cell-to-cell spread and spherical virions for aerosol-based transmission. Matrix Protein 1/M1 Protein, H3N2 (AFM71858, His) is the recombinant Virus-derived Matrix protein 1/M1 protein, expressed by E. coli , with N-His labeled tag. The total length of Matrix Protein 1/M1 Protein, H3N2 (AFM71858, His) is 252 a.a., with molecular weight of ~35 kDa.

基质蛋白 1 (M1) 是病毒复制各个阶段不可或缺的组成部分，从病毒进入和脱壳到病毒颗粒的组装和出芽，发挥着重要作用。M1 与细胞核中的核糖核衣壳 (RNP) 结合被认为可以抑制病毒转录，并且它与病毒 NEP 的相互作用促进复合物的核输出，将其靶向极化上皮细胞顶端质膜上的病毒体组装位点。与 NA 和 HA 的相互作用可能会将 M1 引入脂筏中，尽管它是一种非脂筏相关蛋白。在病毒粒子内，M1 在脂质双层的内侧形成连续的壳，与 RNP 结合。在病毒进入过程中，M2 离子通道酸化内部病毒粒子核心，导致 M1 从 RNP 解离。然后，不含 M1 的 RNP 被转运到细胞核，从而允许病毒转录和复制。此外，M1 决定了病毒粒子的形状，影响其是球形还是丝状。流感的临床分离株通常具有相当大比例的丝状病毒粒子，这对于感染邻近细胞至关重要，而球形病毒粒子更适合宿主生物体之间基于气溶胶的传播^[1][2]。

Virus

E. coli

N-His

AFM71858 (M1-K252)

/

Approximately 35 kDa

Greater than 85% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.