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DiFMUP (Synonyms: 6,8-Difluoro-4-methylumbelliferyl phosphate)

目录号: HY-120166
产品使用指南

DiFMUP 是一种荧光底物,已被广泛用于连续检测磷酸酶活性。

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DiFMUP Chemical Structure

DiFMUP Chemical Structure

CAS No. : 214491-43-7

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Description

DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader[1][2].

In Vitro

DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL)[2].

Molecular Weight

292.13

Formula

C₁₀H₇F₂O₆P

CAS No.

214491-43-7

SMILES

O=P(OC1=C(C(OC2=O)=C(C(C)=C2)C=C1F)F)(O)O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

References
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The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Keywords:

DiFMUP6,8-Difluoro-4-methylumbelliferylOthersfluorogenicsubstratephosphataseactivitiesDIFMUInhibitorinhibitorinhibit

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DiFMUP
目录号:
HY-120166
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