2. Apoptosis
  3. Ferroptosis
  4. FA16

FA16 是一种特异、代谢稳定的铁死亡 (ferroptosis) 诱导剂 (IC50=1.26 μM; HT1080 cells)，是 2-(三氟甲基) 苯并咪唑的衍生物。胱氨酸/谷氨酸反转运蛋白 (system Xc-) 介导了细胞内谷氨酸和细胞外胱氨酸的交换，FA16 通过抑制 system Xc- 行使功能。FA16 也能够在 HepG2 异种移植瘤模型中显著抑制肿瘤生长。

MCE 的所有产品仅用作科学研究或药证申报，我们不为任何个人用途提供产品和服务

FA16 Chemical Structure

FA16 Chemical Structure

1.  客户无需承担相应的运输费用。

2.  同一机构（单位）同一产品试用装仅限申领一次，同一机构（单位）一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户。

规格 是否有货
50 mg   询价  
100 mg   询价  
250 mg   询价  

* Please select Quantity before adding items.

Customer Review

FA16 相关抗体

Top Publications Citing Use of Products

  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

FA16 is a specific ferroptosis inducer (IC50=1.26 μM; HT1080 cells) with metabolic stability, is the derivate of 2-(trifluoromethyl)benzimidazole. FA16 acts by inhibiting cystine/glutamate antiporter (system Xc-), which mediates the exchange of intracellular glutamate and extracellular cystine. FA16 significantly inhibits tumor growth in the HepG2 xenograft model[1].

体外研究
(In Vitro)

FA16 (1 μM; 5 min) 在大鼠和人肝微粒体中具有满意的代谢稳定性[1]。
FA16 (5 μM; 10 h) 诱导 HT1080 细胞中脂质 ROS 积累并抑制谷氨酸释放[1]。
FA16 (5 μM; 24 h) 结果线粒体萎缩，膜密度增大，符合铁衰相关的形态特征[1]。
FA16 (10 μM; 24 h) 诱导的铁死亡能够被铁脱落抑制剂 Fer-1、Trolox 或 DFO 挽救，而凋亡或坏死抑制剂则对其无效[1]。

Parameter Microsomal stability (T1/2 min) Intrinsic clearance (μL/min/ mg protein)
Human 15.6 88.6
Rat 10.4 132.8

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

FA16 相关抗体:

Cell Viability Assay[1]

Cell Line: Human cancer cell lines: Clear-cell renal cell carcinoma cells (786-O), breast cancer cells (MDA-MB-231), cervical cancer cells (HeLa), hepatocellular carcinoma cells (HepG2), melanoma cells (A375), and prostate cancer cells (DU145); Human normal cell lines: cardiomyocytes (AC16), colon mucosal epithelial cells (NCM460), embryonic kidney cells (293T), and hepatic cells (LO2)
Concentration: 0-10 μM
Incubation Time: 48 hours
Result: Inhibited cell growth with IC50s of 0.7 μM (786-O), 4.34 μM (MDA-MB-231), 1.91 μM (HeLa), 1.33 μM (HepG2), 2.31 μM (A375), and 1.64 μM (DU145), respectively.

Immunofluorescence[1]

Cell Line: HT1080 cells
Concentration: 5 μM
Incubation Time: 10 hours
Result: Significantly induced lipid ROS accumulation, as indicated by the great enhancement in green fluorescence intensity.

RT-PCR[1]

Cell Line: HT1080 cells
Concentration: 0.5 μM, 1 μM, and 5 μM
Incubation Time: 6 hours and 18 hours
Result: Increased the system Xc- component SLC7A11, ChaC GSHspecific γ-glutamylcyclotransferase 1 (CHAC1), but little changed GPX4.
体内研究
(In Vivo)

FA16 (15 或 30 mg/kg; 腹腔注射; 隔天给药, 共 21 天) 在 786-O 异种移植物小鼠模型中显著抑制肿瘤生长，安全性好 (不引起体重下降)，还诱导肿瘤组织中发生铁死亡[1]。

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: BALB/c nude mice bearing HepG2 tumors (s.c.)[1]
Dosage: 15 or 30 mg/kg
Administration: Intraperitoneal injection; every other for 21 days
Result: Significantly inhibited tumor growth with a tumor growth inhibition (TGI) value of 47.6% and 77.1% at 15 and 30 mg/ kg, respectively.
分子量

466.52

Formula

C22H25F3N4O2S
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.
纯度 & 产品资料
参考文献

FA16 相关分类

  • 摩尔计算器

  • 稀释计算器

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量   浓度   体积   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FA16FA 16FA-16Ferroptosis2-(trifluoromethyl)benzimidazoleHepG2Inhibitorinhibitorinhibit

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称：

  

* 需求量：

* 客户姓名：

 

* Email：

* 电话：

 

* 公司或机构名称：

   留言给我们：

Bulk Inquiry

Inquiry Information

产品名称：
FA16
目录号：
HY-151964
需求量：

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

   产品名称：

  

* Lot #：

* 客户姓名：

 

* Email：

   电话：

 

* 部门：

* 公司或机构名称：

 

   留言给我们：

Request for HNMR Report

We have received your request and will respond to you as soon as possible.

嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z