2. Academic Validation
  3. Complex pectin metabolism by gut bacteria reveals novel catalytic functions

Complex pectin metabolism by gut bacteria reveals novel catalytic functions

  • Nature. 2017 Apr 6;544(7648):65-70. doi: 10.1038/nature21725.
Didier Ndeh # 1,  Artur Rogowski # 1,  Alan Cartmell # 1,  Ana S Luis # 1,  Arnaud Baslé 1,  Joseph Gray 1,  Immacolata Venditto 1,  Jonathon Briggs 1,  Xiaoyang Zhang 1,  Aurore Labourel 1,  Nicolas Terrapon 2,  Fanny Buffetto 3,  Sergey Nepogodiev 4,  Yao Xiao 5,  Robert A Field 4,  Yanping Zhu 6,  Malcolm A O'Neil 6,  Breeana R Urbanowicz 6,  William S York 6,  Gideon J Davies 7,  D Wade Abbott 8,  Marie-Christine Ralet 3,  Eric C Martens 5,  Bernard Henrissat 2 9 10,  Harry J Gilbert 1
Affiliations

Affiliations

  • 1 Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne NE2 4HH, U.K.
  • 2 Architecture et Fonction des Macromolécules Biologiques, Centre National de la Recherche Scientifique (CNRS), Aix-Marseille University, F-13288 Marseille, France.
  • 3 INRA, UR1268 Biopolymères Interactions Assemblages, 44300 Nantes, France.
  • 4 Department of Biological Chemistry, John Innes Centre Norwich Research Park, Norwich NR4 7UH, UK.
  • 5 Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor, MI, USA.
  • 6 Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Road, Athens, GA 30602, USA.
  • 7 Department of Chemistry, University of York, York YO10 5DD, U.K.
  • 8 Lethbridge Research Centre, Lethbridge, AB, Canada.
  • 9 INRA, USC 1408 AFMB, F-13288 Marseille, France.
  • 10 Department of Biological Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.
  • # Contributed equally.
PMID: 28329766 DOI: 10.1038/nature21725
Abstract

The metabolism of carbohydrate Polymers drives microbial diversity in the human gut microbiota. It is unclear, however, whether Bacterial consortia or single organisms are required to depolymerize highly complex glycans. Here we show that the gut bacterium Bacteroides thetaiotaomicron uses the most structurally complex glycan known: the plant pectic polysaccharide rhamnogalacturonan-II, cleaving all but 1 of its 21 distinct glycosidic linkages. The deconstruction of rhamnogalacturonan-II side chains and backbone are coordinated to overcome steric constraints, and the degradation involves previously undiscovered enzyme families and catalytic activities. The degradation system informs revision of the current structural model of rhamnogalacturonan-II and highlights how individual gut bacteria orchestrate manifold Enzymes to metabolize the most challenging glycan in the human diet.

Figures
Products
嗨！很高兴为您提供帮助！

尊敬的 MCE 客户您好，
请您选择所在区域，我们将转接对应客服为您服务！

热门区域

A

B

C

F

G

H

J

L

N

Q

S

T

X

Y

Z

热 A B C F G H J L N Q S T X Y Z