1. 重组蛋白
  2. Cytokines and Growth Factors
  3. TNF Superfamily
  4. TNF Superfamily Ligands
  5. GITRL/AITRL
  6. AITRL/TNFSF18 蛋白, Human (HEK293, His)

AITRL/TNFSF18 蛋白, Human (HEK293, His)

目录号: HY-P72630
产品使用指南

AITRL,一种 II 型跨膜蛋白,是糖皮质激素诱导的 TNFR 相关蛋白 (GITR) 的配体。当 AITRL 与 GITR 结合时,GITR 可以产生调节 T 细胞增殖和效应子功能的共刺激信号。GITR/GITRL 相互作用在肿瘤、炎症以及自身免疫性疾病的发病中发挥着重要作用。此外,AITRL 还在内皮细胞 (ECs) 的激活中发挥作用,并促进小鼠和人类细胞的粘附,从而增加 STAT-1 磷酸化和粘附分子 (如 VCAM-1 和 ICAM-1) 的表达。AITRL/TNFSF18 蛋白, Human (HEK293, His) 是一种重组人 AITRL (E74-S199),其 C 端带有 6*His 标签,在 HEK293 中表达。

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描述

AITRL, a type II transmembrane protein, is a ligand for glucocorticoid-induced TNFR-related protein (GITR). When AITRL binds to GITR, GITR can produce costimulatory signals that regulate T-cell proliferation and effector functions. GITR/AITRL interaction plays a role in the pathogenesis of tumor, inflammation, as well as autoimmune diseases[1]. Besides, AITRL plays a role in endothelial cells (EC)-activation and increases STAT-1 phosphorylation and the expression of adhesion molecules (VCAM-1, ICAM-1)[2]. AITRL/TNFSF18 Protein, Human (HEK293, His) is a recombinant human AITRL (E74-S199) with C-terminal 6*His tag, which is expressed in HEK293.

研究背景

GITRL (AITRL), a type II transmembrane protein, is a ligand for glucocorticoid-induced TNFR-related protein (GITR). GITR, a member of the TNFR superfamily, is expressed in T cells, natural killer cells and some myeloid cells. And GITRL is mainly expressed on antigen presenting cells (B cells, dendritic cells), macrophages and endothelial cells (ECs)[1].
When GITRL binds to GITR, GITR can produce costimulatory signals that regulate T-cell proliferation and effector functions. The interaction stimulates proliferation and cytokine production of both CD4+ Teff and Treg cells, and drives antitumor activity of CD8+ T cells[3]. Besides, GITRL plays a role in EC-activation and promotes adhesion in both mice and humans, which increases STAT-1 phosphorylation and the augmented expression of adhesion molecules such as VCAM-1 and ICAM-1[2].
Human GITRL shares < 55% common aa identity with mouse. Human GITRL consists of cytoplasmic domain (M1-W27), helical domain (L28-F48), and extracellular domain (L49-S177). Human GITRL is a trimer, but can also be a monomer or assemble in other multimeric structures[4].
GITR/GITRL interaction plays a role in the pathogenesis of tumor, inflammation, as well as autoimmune diseases[1].

体外研究
(In Vitro)

AITRL (human, 48 h) reduces the suppressive capacity of MDSCs on CD4+ T cells (MDSCs induced from healthy donors)[5].

种属

Human

表达系统

HEK293

标签

C-6*His

蛋白编号

Q9UNG2 (E74-S199)

基因 ID

8995  [NCBI]

中文名
重组人 AITRL/TNFSF18 蛋白 (HEK293, His)
同用名
Tumor necrosis factor ligand superfamily member 18; AITRL; hGITRL; TNFSF18; TL6
氨基酸序列

ETAKEPCMAKFGPLPSKWQMASSEPPCVNKVSDWKLEILQNGLYLIYGQVAPNANYNDVAPFEVRLYKNKDMIQTLTNKSKIQNVGGTYELHVGDTIDLIFNSEHQVLKNNTYWGIILLANPQFIS

分子量

14-22 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

AITRL/TNFSF18 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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AITRL/TNFSF18 Protein, Human (HEK293, His)
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