1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Neurotrophic Factors
  4. Neurotrophins/NGF
  5. Nerve Growth Factor-β (Beta-NGF)
  6. Beta-NGF 蛋白, Mouse (120a.a)

Beta-NGF 蛋白, Mouse (120a.a)

目录号: HY-P7661
COA 产品使用指南

Beta-NGF 蛋白, Mouse (120a.a) 在大肠杆菌中表达。Beta-NGF 是 NGF 家族的重要成员,具有 NGF 的生物学活性。

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重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥800 In-stock
50 μg ¥2400 In-stock
100 μg   询价  
250 μg   询价  

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  • 参考文献

描述

Beta-NGF Protein, Mouse (120a.a) expresses in E. coli. Beta-Nerve Growth Factor is an important member of the NGF family, which displays the biological activity of NGF[1].

研究背景

Beta-nerve growth factor is a basic protein of 118 amino acids which acts are a trophic factor for sensory and sympathetic neurons of the peripheral nervous system, and on cholinergic neurons of the anterior basal cerebrum. Beta-nerve growth factor promotes tumor initiation and progression in many human tumors, such as breast, prostate, and oral cancers[1][2].

生物活性

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.3-1.5 ng/ml.

种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

P01139 (S122-G241)

基因 ID

18049  [NCBI]

中文名
重组小鼠 β 神经生长因子
同用名
rMuBeta-NGF; Beta-nerve growth factor; Beta-NGF;
氨基酸序列

SSTHPVFHMGEFSVCDSVSVWVGDKTTATDIKGKEVTVLAEVNINNSVFRQYFFETKCRASNPVESGCRGIDSKHWNSYCTTTHTFVKALTTDEKQAAWRFIRIDTACVCVLSRKATRRG

分子量

Approximately 14.0 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against 20 mM Tris,150 mM NaCl, pH8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O or PBS.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Beta-NGF Protein, Mouse (120a.a)
目录号:
HY-P7661
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