1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens Receptor Proteins Enzymes & Regulators
  3. TGF-beta Superfamily Epithelial cell CD Proteins Receptor Serine/Threonine Kinases Serine/Threonine Kinase Proteins
  4. BMP Receptor ALK-3/CD292
  5. ALK-3
  6. BMPR1A/ALK-3 蛋白, Human (152a.a, HEK293, His-Fc)

BMPR1A/ALK-3 蛋白, Human (152a.a, HEK293, His-Fc)

目录号: HY-P75593
产品使用指南

BMPR1A/ALK-3 蛋白 (ACVRLK3) 是骨形态发生蛋白 I 型受体,在组织中广泛表达。BMPR1A/ALK-3 蛋白介导铁代谢因子 hepcidin 表达,与 GDF5/6 相互作用调节软骨细胞分化和脂肪形成。BMPR1A-ID2/ZEB1-TGFBR2 信号可能是肺动脉高压 (PAH) 和其他内皮间质转换 (EndoMT) 相关血管疾病的潜在靶点。人的 BMPR1A/ALK-3 蛋白全长 532 个氨基酸,具有一个特异识别 BMP 配体的基序 (107-109 a.a.)。重组人 BMPR1A/ALK-3 蛋白 (152a.a, HEK293, His-Fc) 由 HEK293 细胞表达,全长 152 个氨基酸 (M1-R152),带有 C 端 hFc 和 His 标签。

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描述

BMPR1A/ALK-3 protein (ACVRLK3) is the receptor bone morphogenetic protein (BMP) type I receptors, widely expressed in tissues[1]. BMPR1A/ALK-3 protein mediates iron metabolism factor hepcidin expression, interacts with GDF5/6 to regulate chondrocyte differentiation and adipogenesis[2][3]. BMPR1A-ID2/ZEB1-TGFBR2 signaling axis could serve as a potentia target for pulmonary arterial hypertension (PAH) and other endothelial-mesenchymal transition (EndoMT)-related vascular disorders[4]. Human BMPR1A/ALK-3 has a full length of 532 a.a., with a motif (107-109 a.a.) mediating specificity for BMP ligand. BMPR1A/ALK-3 Protein, Human (152a.a, HEK293, His-Fc) is produced in HEK293 cells and has 152 amino acids (M1-R152), with C-terminal hFc- and His-tags.

研究背景

ALK-3 (BMPR1A; ACVRLK3) is the receptor bone morphogenetic protein (BMP) type I receptors, for BMP2, BMP4, GDF5 and GDF6. Among BMP type I receptors, ALK-2 and 3 are widely expressedin tissues, while ALK-1 is more selectively expressed in endothelial cells (ECs)[1]. Hepcidin, the main regulator of iron metabolism, is synthesized and released by hepatocytes in response to increased body iron concentration and inflammation. BMP/ALK/SMAD pathway controls hepcidin expression, while BMP type I receptors ALK-2 and ALK-3 are responsible for iron-dependent hepcidin upregulation and basal hepcidin expression, respectively, to avoid low hepcidin which causes iron overload or high hepcidin levels which induce iron-restricted erythropoiesis[2]. ALK-3 positively regulates chondrocyte differentiation through GDF5 interaction and mediates induction of adipogenesis by GDF6[3]. ALK-3 protein shows function for the initiation of chondrogenesis, for regulating differentiation along the chondrogenic lineage, and for endochondral bone formation[5]. Components of BMP signaling have been implicated in both pathogenesis of pulmonary arterial hypertension (PAH) and endothelial-mesenchymal transition (EndoMT), and BMPR1A is key to maintain endothelial identity and to prevent excessive EndoMT. BMPR1A-ID2/ZEB1-TGFBR2 signaling axis could serve as a potential novel target for PAH and other EndoMT-related vascular disorders[4].

体外研究
(In Vitro)

ALK3-Flag (200 ng/mL; 48 h) synergizes with HFE, results phospho-Smad1/5/8, HA increasing and stimulates hepcidin expression in transfected Hep3B cells[6].

生物活性

Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 mouse osteoblastic cells and the ED50 is typically 0.1-0.3 μg/mL in the presence of 50 ng/mL of human BMP4.

种属

Human

表达系统

HEK293

标签

C-hFc;C-His

蛋白编号

P36894 (M1-R152)

基因 ID

657  [NCBI]

中文名
重组人 BMPR1A/ALK-3 蛋白 (152a.a, HEK293, His-Fc)
同用名
ALK-3; BMPR­IA; Bone morphogenetic protein receptor type-1A; SKR5; CD292
分子量

Approximately 56 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

Lyophilized powder.

组分

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
BMPR1A/ALK-3 Protein, Human (152a.a, HEK293, His-Fc)
目录号:
HY-P75593
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