1. 重组蛋白
  2. CD Antigens
  3. T Cell CD Proteins NK Cell CD Proteins Macrophage CD Proteins Dendritic Cell CD Proteins
  4. CD3e
  5. CD3 epsilon 蛋白, Mouse (P.pastoris, His)

CD3 epsilon 蛋白, Mouse (P.pastoris, His)

目录号: HY-P71723
COA 产品使用指南

CD3 epsilon 蛋白是 T 淋巴细胞上 TCR-CD3 复合物的重要组成部分,对于适应性免疫反应至关重要。APC激活TCR后,CD3E与CD3D、CD3G和CD3Z一起通过ITAM传递信号并激活下游通路。CD3 epsilon 蛋白, Mouse (P.pastoris, His) 是重组的 CD3 epsilon 蛋白,由 P. pastoris 表达,带有 N-6*His 标签。CD3 epsilon 蛋白, Mouse (P.pastoris, His) 全长 86 个氨基酸,分子量约为 ~11.9 kDa。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
Free Sample   Apply now  
5 μg ¥940 In-stock
10 μg ¥1600 In-stock
50 μg ¥4500 In-stock
100 μg   询价  

* Please select Quantity before adding items.

注册 MCE会员完成审核
即刻享有 积分商城 300 专属积分

Top Publications Citing Use of Products
  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The CD3 epsilon protein is an important component of the TCR-CD3 complex on T lymphocytes and is critical for adaptive immune responses. After APC activates TCR, CD3E, together with CD3D, CD3G and CD3Z, transmits signals through ITAM and activates downstream pathways. CD3 epsilon Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived CD3 epsilon protein, expressed by P. pastoris , with N-6*His labeled tag. The total length of CD3 epsilon Protein, Mouse (P.pastoris, His) is 86 a.a., with molecular weight of ~11.9 kDa.

研究背景

CD3 epsilon 蛋白是位于 T 淋巴细胞表面的 TCR-CD3 复合物的组成部分,在适应性免疫反应中发挥着至关重要的作用。抗原呈递细胞 (APC) 激活 T 细胞受体 (TCR) 后,CD3E 与 CD3D、CD3G 和 CD3Z 一起促进 TCR 介导的信号跨细胞膜的传输。所有 CD3 链的胞质结构域均包含基于免疫受体酪氨酸的激活基序 (ITAM),该基序在被 LCK 和 FYN 激酶磷酸化后,触发下游信号传导通路。除了在 T 细胞激活的信号转导中发挥作用外,CD3E 对于 T 细胞的正常发育也是不可或缺的。此外,它通过其胞质区域中存在的内吞序列参与 TCR-CD3 复合物的内化和细胞表面下调。TCR-CD3复合物包含优先与TCRα和TCRβ结合形成三聚体的CD3D/CD3E和CD3G/CD3E异二聚体。六聚体与 CD3Z 同二聚体相互作用,形成 TCR-CD3 复合物。或者,TCRalpha和TCRbeta可以被TCRgamma和TCRdelta替代。CD3E 还与 CD6 和 NCK1 相互作用。这个综合网络强调了 CD3E 在协调 T 细胞反应中的多方面作用。

种属

Mouse

表达系统

P. pastoris

标签

N-6*His

蛋白编号

P22646 (D23-D108)

基因 ID

12501  [NCBI]

蛋白结构
N-term
6*His
CD3 epsilon (D23-D108)
Accession # P22646
C-term
同用名
Cd3eT-cell surface glycoprotein CD3 epsilon chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD antigen CD3e
氨基酸序列

DAENIEYKVSISGTSVELTCPLDSDENLKWEKNGQELPQKHDKHLVLQDFSEVEDSGYYVCYTPASNKNTYLYLKARVCEYCVEVD

分子量

Approximately 11.9 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
CD3 epsilon Protein, Mouse (P.pastoris, His)
目录号:
HY-P71723
需求量: