1. 重组蛋白
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  3. Inhibitory Checkpoint Molecules Stem Cell CD Proteins Platelet CD Proteins Erythrocyte CD Proteins Dendritic Cell CD Proteins Epithelial cell CD Proteins Endothelial cell CD Proteins
  4. Integrin Associated Protein/CD47
  5. CD47 蛋白, Human (HEK293, Fc)

CD47 蛋白, Human (HEK293, Fc)

目录号: HY-P7335
COA 产品使用指南

CD47 蛋白, Human (HEK293, Fc)是在 HEK293 中产生的 C-末端标记 IgG1 Fc 片段的多肽链。CD47 是癌症免疫疗法的重要靶标。

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规格 价格 是否有货 数量
10 μg ¥1200 In-stock
50 μg ¥3200 In-stock
100 μg   询价  

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描述

CD47 Protein, Human (HEK293, Fc) is a polypeptide chain with the C-terminal human IgG1 Fc fragment produced in HEK293 cells. CD47 is a prominent target in cancer immunotherapy.

研究背景

靶向 CD47 是癌症免疫治疗的焦点。阻断 CD47 会触发先天免疫识别和消除癌细胞。CD47/SIRP-α 轴已被确定为先天抗癌免疫的重要调节因子,许多(如果不是全部)恶性肿瘤都会过度表达与吞噬细胞表达的 SIRP-α 结合的受体 CD47[1]

生物活性

2 µg/mL (100 µL/well) of immoblized recombinant human SIRPa-His can bind human CD47-Fc with a linear range of 0.25-185 ng/mL.

种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q08722 (Q19-P139)

基因 ID

961  [NCBI]

蛋白结构
N-term
CD47 (Q19-P139)
Accession # Q08722
hFc
C-term
同用名
rHuCD47, Fc Chimera; MER6; IAP; OA3
氨基酸序列

QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSP

分子量

Approximately 58.0 kDa.

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
CD47 Protein, Human (HEK293, Fc)
目录号:
HY-P7335
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